2006
Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8
Fausther M, Lecka J, Kukulski F, Lévesque S, Pelletier J, Zimmermann H, Dranoff JA, Sévigny J. Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8. AJP Gastrointestinal And Liver Physiology 2006, 292: g785-g795. PMID: 17095758, PMCID: PMC3952495, DOI: 10.1152/ajpgi.00293.2006.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAmino Acid SequenceAnimalsAntigens, CDApyraseCatalysisCell LineChlorocebus aethiopsCloning, MolecularCOS CellsDeoxycholic AcidEnzyme InhibitorsHepatocytesHumansKineticsLiverMolecular Sequence DataNucleotidesRatsRats, Sprague-DawleyRecombinant ProteinsSequence Homology, Amino AcidSodium AzideSubstrate SpecificityConceptsExtracellular nucleotidesSpecific transmembrane receptorsAmino acid sequenceTerminal amino acid sequenceHEK293T cellsCritical liver functionsNTPDase2/CD39L1Cell surface enzymeTransmembrane receptorsAcid sequenceHigh identityCOS-7Recombinant proteinsNTPDase8Molecular identityNucleoside salvageEcto-ATPaseAffigel BlueNTPDase3 expression
1999
Short‐term regulation of bile acid uptake by microfilament‐dependent translocation of rat ntcp to the plasma membrane
Dranoff J, McClure M, Burgstahler A, Denson L, Crawford A, Crawford J, Karpen S, Nathanson M. Short‐term regulation of bile acid uptake by microfilament‐dependent translocation of rat ntcp to the plasma membrane. Hepatology 1999, 30: 223-229. PMID: 10385660, DOI: 10.1002/hep.510300136.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAnimalsBase SequenceBile Acids and SaltsBiological TransportCarcinoma, HepatocellularCarrier ProteinsCell MembraneCyclic AMPCytochalasin DGreen Fluorescent ProteinsHumansKineticsLiver NeoplasmsLuminescent ProteinsMembrane Transport ProteinsModels, MolecularMolecular Sequence DataOpen Reading FramesOrganic Anion Transporters, Sodium-DependentProtein Structure, SecondaryRatsRecombinant Fusion ProteinsSymportersTaurocholic AcidTransfectionTritiumTumor Cells, CulturedConceptsPlasma membraneGreen fluorescence proteinGFP fluorescenceNTCP-GFPPlasma membrane fluorescenceMembrane fluorescenceRat NtcpInhibitor of microfilamentsSecond messenger adenosinePrimary transporterFluorescence proteinNocodazole removalCytochalasin DTransporter activityShort-term regulationAcid uptakeConfocal video microscopyMicrotubule inhibitorsHepG2 cellsTaurocholate cotransportMembraneProteinMicrofilamentsCellsVideo microscopy