2011
Coexpression of ecto-5′-nucleotidase/CD73 with specific NTPDases differentially regulates adenosine formation in the rat liver
Fausther M, Lecka J, Soliman E, Kauffenstein G, Pelletier J, Sheung N, Dranoff JA, Sévigny J. Coexpression of ecto-5′-nucleotidase/CD73 with specific NTPDases differentially regulates adenosine formation in the rat liver. AJP Gastrointestinal And Liver Physiology 2011, 302: g447-g459. PMID: 22135310, PMCID: PMC3287391, DOI: 10.1152/ajpgi.00165.2011.Peer-Reviewed Original ResearchConceptsRat liverRecombinant rat enzymeDistinct fibroblast populationsP2 receptor agonistsSmooth muscle cellsNTPDase2/CD39L1Vascular endothelial cellsFibrotic rat liverCD73 protein expressionSpecific biochemical propertiesPortal fibroblastsReceptor agonistP2 receptorsNormal rat liverFibrotic conditionsPortal spacesInhibitor ADPAdenosine formationMuscle cellsCD73Endothelial cellsTriphosphate diphosphohydrolaseFormation of adenosineProtein expressionEctonucleotidases
2006
Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8
Fausther M, Lecka J, Kukulski F, Lévesque S, Pelletier J, Zimmermann H, Dranoff JA, Sévigny J. Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8. AJP Gastrointestinal And Liver Physiology 2006, 292: g785-g795. PMID: 17095758, PMCID: PMC3952495, DOI: 10.1152/ajpgi.00293.2006.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAmino Acid SequenceAnimalsAntigens, CDApyraseCatalysisCell LineChlorocebus aethiopsCloning, MolecularCOS CellsDeoxycholic AcidEnzyme InhibitorsHepatocytesHumansKineticsLiverMolecular Sequence DataNucleotidesRatsRats, Sprague-DawleyRecombinant ProteinsSequence Homology, Amino AcidSodium AzideSubstrate SpecificityConceptsExtracellular nucleotidesSpecific transmembrane receptorsAmino acid sequenceTerminal amino acid sequenceHEK293T cellsCritical liver functionsNTPDase2/CD39L1Cell surface enzymeTransmembrane receptorsAcid sequenceHigh identityCOS-7Recombinant proteinsNTPDase8Molecular identityNucleoside salvageEcto-ATPaseAffigel BlueNTPDase3 expression
2002
The ecto‐nucleoside triphosphate diphosphohydrolase NTPDase2/CD39L1 is expressed in a novel functional compartment within the liver
Dranoff JA, Kruglov EA, Robson SC, Braun N, Zimmermann H, Sévigny J. The ecto‐nucleoside triphosphate diphosphohydrolase NTPDase2/CD39L1 is expressed in a novel functional compartment within the liver. Hepatology 2002, 36: 1135-1144. PMID: 12395323, DOI: 10.1053/jhep.2002.36823.Peer-Reviewed Original ResearchConceptsIntrahepatic bile ductsExtracellular nucleotidesBile ductDiverse biological functionsBlot analysisEcto-nucleoside triphosphate diphosphohydrolasesNTPDase2/CD39L1Portal fibroblastsNorthern blot analysisCellular compartmentsBiological functionsPotential regulatorConfocal immunofluorescenceWestern blot analysisHepatic blood flowBile duct epitheliumReverse transcription-polymerase chain reactionFunctional assaysTriphosphate diphosphohydrolasesImmunoelectron microscopyFunctional compartmentsHepatic central veinNucleotidesNTPDase1NTPDase2
2001
Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia
Dranoff J, Masyuk A, Kruglov E, LaRusso N, Nathanson M. Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia. AJP Gastrointestinal And Liver Physiology 2001, 281: g1059-g1067. PMID: 11557527, DOI: 10.1152/ajpgi.2001.281.4.g1059.Peer-Reviewed Original ResearchConceptsP2Y receptor subtypesP2Y receptorsReceptor subtypesIntrahepatic bile duct unitsATP receptorsBile duct epitheliumMultiple P2Y receptor subtypesRat bile duct epitheliaBile duct unitsImportant regulatorLuminal ATPLuminal perfusionMolecular subtypesBile secretionDuct epitheliumBasolateral additionExtracellular nucleotidesDuctular secretionSubtypesCytosolic Ca2Duct unitsRT-PCRCholangiocytesReceptorsSecretion