2011
Coexpression of ecto-5′-nucleotidase/CD73 with specific NTPDases differentially regulates adenosine formation in the rat liver
Fausther M, Lecka J, Soliman E, Kauffenstein G, Pelletier J, Sheung N, Dranoff JA, Sévigny J. Coexpression of ecto-5′-nucleotidase/CD73 with specific NTPDases differentially regulates adenosine formation in the rat liver. AJP Gastrointestinal And Liver Physiology 2011, 302: g447-g459. PMID: 22135310, PMCID: PMC3287391, DOI: 10.1152/ajpgi.00165.2011.Peer-Reviewed Original ResearchConceptsRat liverRecombinant rat enzymeDistinct fibroblast populationsP2 receptor agonistsSmooth muscle cellsNTPDase2/CD39L1Vascular endothelial cellsFibrotic rat liverCD73 protein expressionSpecific biochemical propertiesPortal fibroblastsReceptor agonistP2 receptorsNormal rat liverFibrotic conditionsPortal spacesInhibitor ADPAdenosine formationMuscle cellsCD73Endothelial cellsTriphosphate diphosphohydrolaseFormation of adenosineProtein expressionEctonucleotidases
2002
Isolation of Primary Rat Liver Fibroblasts
Kruglov EA, Jain D, Dranoff JA. Isolation of Primary Rat Liver Fibroblasts. Journal Of Investigative Medicine 2002, 50: 179. PMID: 12033282, DOI: 10.2310/6650.2002.33431.Peer-Reviewed Original ResearchConceptsLiver fibroblastsProcollagen-1 mRNAReverse transcription-polymerase chain reactionRat liverTranscription-polymerase chain reactionDistinct liver cell populationsSmooth muscle actinSmooth muscle cellsStandard cell culture methodsLiver cell populationsRole of fibroblastsVon Willebrand factorPolymerase chain reactionStellate cellsProcollagen 1Muscle actinCell markersMuscle cellsLiver physiologyAppearance of cellsWillebrand factorFibroblast morphologyChain reactionLiver researchCell populations