2007
Succinate is a paracrine signal for liver damage
Correa PR, Kruglov EA, Thompson M, Leite MF, Dranoff JA, Nathanson MH. Succinate is a paracrine signal for liver damage. Journal Of Hepatology 2007, 47: 262-269. PMID: 17451837, PMCID: PMC1986575, DOI: 10.1016/j.jhep.2007.03.016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFluorescent Antibody TechniqueIn Vitro TechniquesInfusions, IntravenousIschemiaLiverLiver DiseasesMaleParacrine CommunicationPerfusionPortal VeinPressureRatsRats, Sprague-DawleyReceptors, G-Protein-CoupledReverse Transcriptase Polymerase Chain ReactionSignal TransductionSuccinic AcidTissue DistributionConceptsHepatic stellate cellsSuccinate receptorParacrine signalsStellate cell activationStellate cellsCell expression systemTime-lapse imagingRelease of succinateCell activationCytosolic Ca2Effect of succinatePrimary hepatic stellate cellsHepatic cell typesExpression systemQuiescent hepatic stellate cellsConfocal immunofluorescencePhysiological roleIschemic hepatocytesCell typesBiochemical assaysSingle cellsLiver damageBACKGROUND/Western blotCAMP production
2006
Adenosine inhibits cytosolic calcium signals and chemotaxis in hepatic stellate cells
Hashmi AZ, Hakim W, Kruglov EA, Watanabe A, Watkins W, Dranoff JA, Mehal WZ. Adenosine inhibits cytosolic calcium signals and chemotaxis in hepatic stellate cells. AJP Gastrointestinal And Liver Physiology 2006, 292: g395-g401. PMID: 17053161, PMCID: PMC3224076, DOI: 10.1152/ajpgi.00208.2006.Peer-Reviewed Original ResearchConceptsCytosolic Ca2Collagen I mRNATGF-beta productionHepatic stellate cell biologyLX-2 cellsEffects of adenosineHepatic stellate cellsSite of injuryI mRNAElevated tissue levelsDose-dependent mannerHigh adenosine concentrationsStellate cell biologyAdenylate cyclase inhibitorActivation markersLiver fibrosisTissue injuryHSC chemotaxisStellate cellsCyclase inhibitorAdenosine concentrationTranswell systemInhibited increasesCellular hypoxiaTissue levels
2001
Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia
Dranoff J, Masyuk A, Kruglov E, LaRusso N, Nathanson M. Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia. AJP Gastrointestinal And Liver Physiology 2001, 281: g1059-g1067. PMID: 11557527, DOI: 10.1152/ajpgi.2001.281.4.g1059.Peer-Reviewed Original ResearchConceptsP2Y receptor subtypesP2Y receptorsReceptor subtypesIntrahepatic bile duct unitsATP receptorsBile duct epitheliumMultiple P2Y receptor subtypesRat bile duct epitheliaBile duct unitsImportant regulatorLuminal ATPLuminal perfusionMolecular subtypesBile secretionDuct epitheliumBasolateral additionExtracellular nucleotidesDuctular secretionSubtypesCytosolic Ca2Duct unitsRT-PCRCholangiocytesReceptorsSecretion
1999
Expression and subcellular localization of the ryanodine receptor in rat pancreatic acinar cells.
Leite MF, Dranoff JA, Gao L, Nathanson MH. Expression and subcellular localization of the ryanodine receptor in rat pancreatic acinar cells. Biochemical Journal 1999, 337 ( Pt 2): 305-9. PMID: 9882629, PMCID: PMC1219966, DOI: 10.1042/0264-6021:3370305.Peer-Reviewed Original ResearchConceptsPancreatic acinar cellsNon-excitable cell typesCell typesCell types resultsAcinar cellsNon-excitable cellsRyanodine receptorSubcellular localizationPolarized epitheliumReverse transcription-PCRRat pancreatic acinar cellsTrisphosphate receptorCo-ordinated releaseType 2 RyRExcitable cellsRelease pathwayTranscription-PCRPrincipal Ca2Release channelApical regionCytosolic Ca2CellsFundamental roleRyRsPropagation of Ca2Expression and subcellular localization of the ryanodine receptor in rat pancreatic acinar cells
LEITE M, DRANOFF J, Ling G, NATHANSON M. Expression and subcellular localization of the ryanodine receptor in rat pancreatic acinar cells. Biochemical Journal 1999, 337: 305-309. DOI: 10.1042/bj3370305.Peer-Reviewed Original ResearchPancreatic acinar cellsNon-excitable cell typesCell typesCell types resultsAcinar cellsNon-excitable cellsRyanodine receptorSubcellular localizationPolarized epitheliumReverse transcription-PCRRat pancreatic acinar cellsTrisphosphate receptorCo-ordinated releaseType 2 RyRExcitable cellsRelease pathwayTranscription-PCRPrincipal Ca2Release channelApical regionCytosolic Ca2CellsFundamental roleRyRsPropagation of Ca2