2009
Intracellular calcium signals regulate growth of hepatic stellate cells via specific effects on cell cycle progression
Soliman EM, Rodrigues MA, Gomes DA, Sheung N, Yu J, Amaya MJ, Nathanson MH, Dranoff JA. Intracellular calcium signals regulate growth of hepatic stellate cells via specific effects on cell cycle progression. Cell Calcium 2009, 45: 284-292. PMID: 19131107, PMCID: PMC3018528, DOI: 10.1016/j.ceca.2008.11.006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium SignalingCalcium-Calmodulin-Dependent Protein Kinase Type 2Cdc25 PhosphatasesCell CompartmentationCell CycleCell NucleusCell ProliferationChelating AgentsCytosolEgtazic AcidEnzyme InhibitorsG2 PhaseHepatocytesHumansIntracellular SpaceParvalbuminsPhosphorylationProtein TransportRatsSubcellular FractionsConceptsHepatic stellate cellsLiver fibrosisStellate cellsImmortalized human hepatic stellate cellsHSC growthHuman hepatic stellate cellsRat hepatic stellate cellsPrimary rat hepatic stellate cellsIntracellular calcium signalsCdc25C phosphorylationPharmacological therapyCalmodulin kinase IIImportant mediatorCell cycle progressionHSC proliferationCell proliferationLogical targetCalcium signalsAdenoviral constructDownstream intracellularG2/M checkpointCaMK IIFibrosisBlockadeParvalbumin
2005
Portal Fibroblasts Regulate the Proliferation of Bile Duct Epithelia via Expression of NTPDase2*
Jhandier MN, Kruglov EA, Lavoie É, Sévigny J, Dranoff JA. Portal Fibroblasts Regulate the Proliferation of Bile Duct Epithelia via Expression of NTPDase2*. Journal Of Biological Chemistry 2005, 280: 22986-22992. PMID: 15799977, DOI: 10.1074/jbc.m412371200.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAnimalsBile DuctsBromodeoxyuridineCell ProliferationCholangiocarcinomaCholestasisCoculture TechniquesDNA, ComplementaryEpithelial CellsFibroblastsHumansLiverMaleMicroscopy, ConfocalMicroscopy, FluorescenceModels, BiologicalRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionRNA, Small InterferingSignal TransductionTransfectionConceptsBile ductular proliferationExpression of NTPDase2Portal fibroblastsDuctular proliferationBile duct epitheliumNTPDase2 expressionMz-ChA-1 cellsPortal myofibroblastsP2Y receptorsDuct epitheliumBile duct-ligated ratsCell proliferationDuct-ligated ratsReal-time reverse transcription PCRQuantitative real-time reverse transcription PCRHuman cholangiocarcinoma cellsNovel co-culture modelMz-ChA-1 human cholangiocarcinoma cellsNucleotidase apyraseP2Y activationCo-culture modelObstructive cholestasisReverse transcription-PCRPathologic alterationsEpithelial proliferation