2024
Lytic bacteriophages induce the secretion of antiviral and proinflammatory cytokines from human respiratory epithelial cells
Zamora P, Reidy T, Armbruster C, Sun M, Van Tyne D, Turner P, Koff J, Bomberger J. Lytic bacteriophages induce the secretion of antiviral and proinflammatory cytokines from human respiratory epithelial cells. PLOS Biology 2024, 22: e3002566. PMID: 38652717, PMCID: PMC11037538, DOI: 10.1371/journal.pbio.3002566.Peer-Reviewed Original ResearchConceptsLytic phagesLytic bacteriophagesPhage therapyAirway epithelial cellsPseudomonas aeruginosa phagesEpithelial cellsMultidrug resistanceAirway epitheliumCystic fibrosisProinflammatory cytokinesHuman respiratory epithelial cellsPhage exposurePhage familiesMammalian cell responsesHuman airway epithelial cellsInternalized phageTreat multidrug-resistantPhageBacterial isolatesTranscriptional profilesRespiratory epithelial cellsHuman hostChronic respiratory disordersBacterial biofilmsBacteriophage
2023
Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epithelia
Castaneda D, Jangra S, Yurieva M, Martinek J, Callender M, Coxe M, Choi A, Diego J, Lin J, Wu T, Marches F, Chaussabel D, Yu P, Salner A, Aucello G, Koff J, Hudson B, Church S, Gorman K, Anguiano E, García-Sastre A, Williams A, Schotsaert M, Palucka K. Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epithelia. IScience 2023, 26: 107374. PMID: 37520727, PMCID: PMC10374611, DOI: 10.1016/j.isci.2023.107374.Peer-Reviewed Original ResearchHuman air-liquid interface culturesAir-liquid interface culturesNucleoprotein expressionAirway epithelial cellsSARS-CoV-2 variant infectionResponse to virusesUnmet medical needCSF3 expressionInterface culturesImmune responseEpithelial cellsDay 4Severe diseaseVariant infectionBasal cellsTranscriptional signatureSARS-CoV-2InfectionApical cellsEarly responseMedical needSARS-CoV-2 virusEpitheliaVirusPost-infection
2014
EGFR activation suppresses respiratory virus-induced IRF1-dependent CXCL10 production
Kalinowski A, Ueki I, Min-Oo G, Ballon-Landa E, Knoff D, Galen B, Lanier LL, Nadel JA, Koff JL. EGFR activation suppresses respiratory virus-induced IRF1-dependent CXCL10 production. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2014, 307: l186-l196. PMID: 24838750, PMCID: PMC4101792, DOI: 10.1152/ajplung.00368.2013.Peer-Reviewed Original ResearchConceptsRespiratory viral infectionsRespiratory syncytial virusCXCL10 productionRespiratory virusesViral infectionIFN regulatory factorAirway epitheliumEGFR activationChronic obstructive pulmonary diseaseEpithelial cellsExacerbation of asthmaObstructive pulmonary diseaseRecruitment of lymphocytesAdaptive immune responsesAirway epithelial cellsNADPH oxidase activationInhibition of EGFRVirus-infected cellsHuman viral pathogensPulmonary diseaseSyncytial virusEpithelial productionIL-8Viral pneumoniaEpidermal growth factor receptor (EGFR) activation
2008
Multiple TLRs activate EGFR via a signaling cascade to produce innate immune responses in airway epithelium
Koff JL, Shao MX, Ueki IF, Nadel JA. Multiple TLRs activate EGFR via a signaling cascade to produce innate immune responses in airway epithelium. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2008, 294: l1068-l1075. PMID: 18375743, DOI: 10.1152/ajplung.00025.2008.Peer-Reviewed Original ResearchMeSH KeywordsADAM ProteinsADAM17 ProteinBronchiCells, CulturedDual OxidasesErbB ReceptorsHumansImmunity, InnateInterleukin-8NADPH OxidasesReactive Oxygen SpeciesRespiratory MucosaRNA, Small InterferingSignal TransductionToll-Like ReceptorsTransforming Growth Factor alphaVascular Endothelial Growth Factor AConceptsToll-like receptorsTNF-alpha converting enzymeInnate immune responseMultiple Toll-like receptorsIL-8Immune responseTGF-alphaVEGF productionTLR ligandsAirway epitheliumEpithelial cellsCertain innate immune responsesEGF receptorMultiple TLR ligandsAirway epithelial surfaceAirway epithelial cell lineNormal human bronchial epithelial cellsEpithelial cell productionAirway epithelial cellsHuman bronchial epithelial cellsNADPH oxidase inhibitorBronchial epithelial cellsEpithelial cell lineReactive oxygen species (ROS) scavengerEpithelial production
2007
Regulation of interleukin-8 via an airway epithelial signaling cascade
Nakanaga T, Nadel JA, Ueki IF, Koff JL, Shao MX. Regulation of interleukin-8 via an airway epithelial signaling cascade. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2007, 292: l1289-l1296. PMID: 17220369, DOI: 10.1152/ajplung.00356.2006.Peer-Reviewed Original ResearchConceptsIL-8 productionIL-8 expressionHuman airway epithelial NCI-H292 cellsEGF receptorNeutrophil chemoattractant interleukinEpithelial cellsNCI-H292 cellsAirway epithelial cellsModel inflammatory stimulusNegative bacterial lipopolysaccharideDual oxidase 1MAP kinases ERK1/2IL-8Tumor necrosisInterleukin-8Immune organsInflammatory stimuliMucin productionHuman airwaysAirwayEpithelial defenseTACE activationBacterial infectionsHost defenseConverting enzyme
2006
Pseudomonas Lipopolysaccharide Accelerates Wound Repair via Activation of a Novel Epithelial Cell Signaling Cascade
Koff JL, Shao MX, Kim S, Ueki IF, Nadel JA. Pseudomonas Lipopolysaccharide Accelerates Wound Repair via Activation of a Novel Epithelial Cell Signaling Cascade. The Journal Of Immunology 2006, 177: 8693-8700. PMID: 17142770, DOI: 10.4049/jimmunol.177.12.8693.Peer-Reviewed Original ResearchConceptsTNF-alpha converting enzymeEGFR phosphorylationOxidase 1Wound repairNCI-H292 human airway epithelial cellsEpidermal growth factor receptor (EGFR) activationGrowth factor receptor activationAirway epithelial cellsEpithelial cellsCell signaling cascadesNormal human bronchial epithelial cellsTLR-4Airway epitheliumHuman bronchial epithelial cellsReactive oxygen species (ROS) scavengerPhosphorylation pathwaySignaling cascadesEssential functionsBronchial epithelial cellsEGFR ligandsHuman airway epithelial cellsChronic airway diseasesOxygen species scavengersPseudomonas bacteriaDual oxidase 1