2016
Functional and molecular identification of a TASK-1 potassium channel regulating chloride secretion through CFTR channels in the shark rectal gland: implications for cystic fibrosis
Telles CJ, Decker SE, Motley WW, Peters AW, Mehr AP, Frizzell RA, Forrest JN. Functional and molecular identification of a TASK-1 potassium channel regulating chloride secretion through CFTR channels in the shark rectal gland: implications for cystic fibrosis. American Journal Of Physiology - Cell Physiology 2016, 311: c884-c894. PMID: 27653983, PMCID: PMC5206301, DOI: 10.1152/ajpcell.00030.2016.Peer-Reviewed Original ResearchConceptsShark rectal glandRectal glandCFTR channelsConfocal immunofluorescent microscopyChloride secretionTASK-1 potassium channelFour-transmembraneApical chloride secretionMammalian lung tissuePotassium channelsGenomic walkingTwo-electrode voltage clampingTASK-1 proteinRapid amplificationDegenerate primersMolecular identificationMolecular identityHuman biologyAmino acidsXenopus oocytesHuman TASK-1ProteinTASK-1 channelsHuman airway cellsIdentical current-voltage relationships
2006
Shark rectal gland vasoactive intestinal peptide receptor: cloning, functional expression, and regulation of CFTR chloride channels
Bewley MS, Pena JT, Plesch FN, Decker SE, Weber GJ, Forrest JN. Shark rectal gland vasoactive intestinal peptide receptor: cloning, functional expression, and regulation of CFTR chloride channels. AJP Regulatory Integrative And Comparative Physiology 2006, 291: r1157-r1164. PMID: 16728467, DOI: 10.1152/ajpregu.00078.2006.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell Cycle ProteinsChloridesCloning, MolecularConserved SequenceCystic Fibrosis Transmembrane Conductance RegulatorDogfishEndodeoxyribonucleasesGene Expression RegulationIn Vitro TechniquesMaleMolecular Sequence DataOocytesPatch-Clamp TechniquesPhylogenyReceptors, Vasoactive Intestinal PeptideReverse Transcriptase Polymerase Chain ReactionSalt GlandVasoactive Intestinal PeptideXenopus laevisConceptsShark rectal glandCFTR chloride channelRectal glandVasoactive intestinal peptideChloride channelsN-glycosylation sitesG protein-coupled receptorsUnique G protein-coupled receptorProtein-coupled receptorsChloride conductanceCysteine residuesNH2 terminusChloride secretionFunctional expressionGrowth hormone-releasing hormoneCFTR mRNADogfish sharkAmino acidsPeptide histidine isoleucine amideRelative potencyXenopus oocytesHormone-releasing hormoneHistidine isoleucine amideQuantitative PCRMicroeq x
2005
Mercury and zinc differentially inhibit shark and human CFTR orthologues: involvement of shark cysteine 102
Weber GJ, Mehr AP, Sirota JC, Aller SG, Decker SE, Dawson DC, Forrest JN. Mercury and zinc differentially inhibit shark and human CFTR orthologues: involvement of shark cysteine 102. American Journal Of Physiology - Cell Physiology 2005, 290: c793-c801. PMID: 16236827, DOI: 10.1152/ajpcell.00203.2005.Peer-Reviewed Original Research