Featured Publications
Light microscopy of proteins in their ultrastructural context
M’Saad O, Bewersdorf J. Light microscopy of proteins in their ultrastructural context. Nature Communications 2020, 11: 3850. PMID: 32737322, PMCID: PMC7395138, DOI: 10.1038/s41467-020-17523-8.Peer-Reviewed Original ResearchConceptsUltrastructural contextLocal protein densityConventional confocal microscopeCellular nanoarchitectureOptical contrastSpecific proteinsElectron microscopyFluorescence microscopyLight microscopyProteinBulk labelingProtein densityConfocal microscopeStandard light microscopyIntracellular spaceMicroscopyProteomeCellsNanoscaleMicroscopeNew principleDiscoveryLabelingPhysical expansionDensity
2020
Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging
Zhang Y, Schroeder LK, Lessard MD, Kidd P, Chung J, Song Y, Benedetti L, Li Y, Ries J, Grimm JB, Lavis LD, De Camilli P, Rothman JE, Baddeley D, Bewersdorf J. Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging. Nature Methods 2020, 17: 225-231. PMID: 31907447, PMCID: PMC7028321, DOI: 10.1038/s41592-019-0676-4.Peer-Reviewed Original ResearchConceptsSuper-resolution microscopeProtein complexesNanoscale resolutionSubcellular architectureMammalian cellsCellular organellesPlasma membraneGolgi apparatusEndoplasmic reticulumElectron microscopyThree-dimensional imagingFluorescence approachMulticolor imagingSpatial organizationLocalization precisionFluorescence imagingMolecular specificityFluorescence imagesMicroscopeFluorescent probeOrganellesReticulumMicroscopyImagingMembrane