Neocortical hyperexcitability in a human case of tuberous sclerosis complex and mice lacking neuronal expression of TSC1
Wang Y, Greenwood J, Calcagnotto M, Kirsch H, Barbaro N, Baraban S. Neocortical hyperexcitability in a human case of tuberous sclerosis complex and mice lacking neuronal expression of TSC1. Annals Of Neurology 2007, 61: 139-152. PMID: 17279540, DOI: 10.1002/ana.21058.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsBlotting, WesternElectric StimulationElectrophysiologyEpilepsyExcitatory Postsynaptic PotentialsFemaleHumansImmunohistochemistryIn Vitro TechniquesInhibitory Postsynaptic PotentialsMiceMice, KnockoutNeocortexNeuronsPatch-Clamp TechniquesProto-Oncogene Proteins c-fosReceptors, AMPASynapsesTuberous SclerosisTuberous Sclerosis Complex 1 ProteinTumor Suppressor ProteinsConceptsTuberous sclerosis complexCortical tubersSynaptic currentsHuman tissue samplesMethyl-4-isoxazolepropionate (AMPA) receptorsWhole-cell patch-clamp recordingsAge-matched control miceTSC brain tissueInhibitory postsynaptic currentsTissue samplesNeocortical brain slicesExtracellular field recordingsInhibitory synaptic currentsDetailed anatomic studyAbnormal electrical dischargesExcitatory synaptic currentsPatch-clamp recordingsGross anatomic defectsLoss of laminationNeuronal firing patternsWestern blot analysisBrain tissue sectionsControl micePostsynaptic currentsAdditional mice