2022
Counting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations
Liao M, Kuo Y, Howard J. Counting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations. Molecular Biology Of The Cell 2022, 33: ar48. PMID: 35323029, PMCID: PMC9265152, DOI: 10.1091/mbc.e21-12-0618.Peer-Reviewed Original ResearchConceptsEnd-binding protein 1Spinning-disk confocal microscopyConfocal microscopySingle-molecule imagingComplex biological phenomenaFly larvaeLiving cellsCell surfaceMicrotubule endsBiological phenomenaProtein 1Brightness of fluorophoresCytoplasmic concentrationEpifluorescence microscopeBiological systemsSensory neuronsCellsSingle moleculesAbsolute numberMolecular numberTissueLarvaeMicrotubulesEGFPProtein
2008
XMAP215 Is a Processive Microtubule Polymerase
Brouhard GJ, Stear JH, Noetzel TL, Al-Bassam J, Kinoshita K, Harrison SC, Howard J, Hyman AA. XMAP215 Is a Processive Microtubule Polymerase. Cell 2008, 132: 79-88. PMID: 18191222, PMCID: PMC2311386, DOI: 10.1016/j.cell.2007.11.043.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesBiological AssayCatalytic DomainCell DifferentiationCell EnlargementCell LineCytoskeletonDiffusionDimerizationFetal ProteinsForminsGreen Fluorescent ProteinsMicrofilament ProteinsMicroscopy, FluorescenceMicrotubule-Associated ProteinsMicrotubulesNuclear ProteinsPolymersProtein BindingProtein Structure, TertiaryProtein TransportSpodopteraSus scrofaTubulinXenopus laevisXenopus ProteinsConceptsFamily of proteinsSingle-molecule assaysActin polymerasesMicrotubule cytoskeletonProcessive polymeraseTubulin subunit additionDynamic microtubulesMicrotubule polymeraseMicrotubule growthCytoskeletal polymersXMAP215Plus endsMicrotubule latticePolymeraseSubunit additionCell proliferationFree tubulinTubulin dimersMicrotubulesCommon mechanismFast growthTip trackingGrowthCytoskeletonRapid assembly
2006
Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner
Varga V, Helenius J, Tanaka K, Hyman AA, Tanaka TU, Howard J. Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nature Cell Biology 2006, 8: 957-962. PMID: 16906145, DOI: 10.1038/ncb1462.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsKinesinsMicrotubule-Associated ProteinsMicrotubulesMolecular Motor ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSpindle ApparatusSwineTubulinConceptsKinesin-8 proteinsLength-dependent depolymerizationYeast kinesin-8Length-dependent mannerMetazoan cellsMicrotubule cytoskeletonMitotic spindleDisassemble microtubulesSpindle lengthKinesin-8Kinesin-13Kip3pPlus endsMicrotubulesConstituent microtubulesLong microtubulesNew mechanismCytoskeletonFungiKinesinProteinSpindleCells