Label‐free high‐speed wide‐field imaging of single microtubules using interference reflection microscopy
MAHAMDEH M, SIMMERT S, LUCHNIAK A, SCHÄFFER E, HOWARD J. Label‐free high‐speed wide‐field imaging of single microtubules using interference reflection microscopy. Journal Of Microscopy 2018, 272: 60-66. PMID: 30044498, PMCID: PMC6486835, DOI: 10.1111/jmi.12744.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleFluorescenceLightMicroscopy, FluorescenceMicroscopy, InterferenceMicrotubulesConceptsDifferential interference contrastReflection microscopyInterference contrastCostly optical componentsWide-field imagingLabel-free imagingHollow cylindrical beamsDark-field condenserInterference contrast imagesDifferential interference contrast (DIC) imagesEfficient fluorescent labelingOptical componentsInterference reflection microscopyOptical misalignmentMicroscope modificationsProtein building blocksNomarski prismOptical qualityHigh-speed imagingNumber of drawbacksFree imagingLight interferenceCylindrical beamsContrast imagesHigh contrast