Counting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations
Liao M, Kuo Y, Howard J. Counting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations. Molecular Biology Of The Cell 2022, 33: ar48. PMID: 35323029, PMCID: PMC9265152, DOI: 10.1091/mbc.e21-12-0618.Peer-Reviewed Original ResearchConceptsEnd-binding protein 1Spinning-disk confocal microscopyConfocal microscopySingle-molecule imagingComplex biological phenomenaFly larvaeLiving cellsCell surfaceMicrotubule endsBiological phenomenaProtein 1Brightness of fluorophoresCytoplasmic concentrationEpifluorescence microscopeBiological systemsSensory neuronsCellsSingle moleculesAbsolute numberMolecular numberTissueLarvaeMicrotubulesEGFPProtein