2022
Predicting the locations of force-generating dyneins in beating cilia and flagella
Howard J, Chasteen A, Ouyang X, Geyer VF, Sartori P. Predicting the locations of force-generating dyneins in beating cilia and flagella. Frontiers In Cell And Developmental Biology 2022, 10: 995847. PMID: 36303602, PMCID: PMC9592896, DOI: 10.3389/fcell.2022.995847.Peer-Reviewed Original ResearchDynein motorsDynein motor proteinsCryogenic electron microscopyMotor proteinsCylindrical organellesDyneinSperm flagellaConformational statesFlagellaAxonemeMotile structuresCiliaThree-dimensional architectureSingle pairOrganellesATPasesIndividual motor activityProteinMolecular mechanics approachActivity changesLarge numberCellsKey questions
2021
Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism
Rao Q, Han L, Wang Y, Chai P, Kuo YW, Yang R, Hu F, Yang Y, Howard J, Zhang K. Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism. Nature Structural & Molecular Biology 2021, 28: 799-810. PMID: 34556869, PMCID: PMC8500839, DOI: 10.1038/s41594-021-00656-9.Peer-Reviewed Original ResearchConceptsOuter arm dyneinMicrotubule doubletsDistinct microtubule-binding domainsHigh-resolution structuresAction of dyneinsMicrotubule-binding domainNative tracksATP hydrolysisDynein motorsHydrolyze ATPConformational changesNucleotide cycleMotor coordination mechanismATP turnoverDyneinHead interactionsMechanical forces