1989
[32] Immunoprecipitation of ribonucleoproteins using autoantibodies
Steitz J. [32] Immunoprecipitation of ribonucleoproteins using autoantibodies. Methods In Enzymology 1989, 180: 468-481. PMID: 2693908, DOI: 10.1016/0076-6879(89)80118-1.Peer-Reviewed Original ResearchMeSH KeywordsAntigen-Antibody ComplexAutoantibodiesCells, CulturedHumansPhosphorus RadioisotopesRadioisotope Dilution TechniqueRibonucleoproteinsSulfur RadioisotopesConceptsSmall ribonucleoproteinImmunoprecipitation procedureTissue culture cellsRNP complexesRNA degradationLaemmli gelsRibonucleoproteinCulture cellsImmunoprecipitationWestern blottingProteinMouse ascitesRNAsCell culture supernatantsCulture supernatantsPhosphoproteinMonoclonal antibodiesRNAImmunoprecipitatesTissueRibonuclease levelsHigh backgroundMouse monoclonal autoantibodyBlottingMonoclonal autoantibodies
1984
The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies.
Pettersson I, Hinterberger M, Mimori T, Gottlieb E, Steitz JA. The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies. Journal Of Biological Chemistry 1984, 259: 5907-5914. PMID: 6232278, DOI: 10.1016/s0021-9258(18)91101-4.Peer-Reviewed Original ResearchConceptsSm small nuclear ribonucleoproteinsSmall nuclear ribonucleoproteinNuclear ribonucleoproteinProtein componentsAnti-Sm patient seraMultiple protein componentsSnRNP proteinsU6 RNAMammalian cellsDistinct polypeptidesAnti-Sm monoclonal antibodyRNA immunoprecipitationAnti-Sm autoantibodiesSnRNP polypeptidesProtein compositionSnRNP particlesProtein BMonoclonal antibodiesRibonucleoproteinIntriguing questionPolypeptideProteinBlot resultsMouse monoclonal antibodyEntire collection
1983
Sequential association of nucleolar 7-2 RNA with two different autoantigens.
Hashimoto C, Steitz JA. Sequential association of nucleolar 7-2 RNA with two different autoantigens. Journal Of Biological Chemistry 1983, 258: 1379-1382. PMID: 6185483, DOI: 10.1016/s0021-9258(18)32990-9.Peer-Reviewed Original ResearchStructure and Function of Small Ribonucleoproteins from Eukaryotic Cells
Steitz JA, Berg C, Gottlieb E, Hardin JA, Hashimoto C, Hendrick JP, Hinterberger M, Krikeles M, Lerner MR, Mount SM, Pettersson I, Rinke J, Rosa M, Wolin S. Structure and Function of Small Ribonucleoproteins from Eukaryotic Cells. 1983, 12: 309-317. PMID: 7166547, DOI: 10.1016/b978-0-12-501650-6.50023-7.Peer-Reviewed Original ResearchMeSH KeywordsAntigen-Antibody ComplexAutoantibodiesHumansLupus Erythematosus, SystemicNucleoproteinsRibonucleoproteinsRNA Polymerase IIITranscription, GeneticConceptsSmall ribonucleoproteinLa proteinRNA polymerase III transcription factorSmall RNA-protein complexesDrosophila U1 RNAU1 small nuclearRNA-protein complexesHnRNA splicingEukaryotic cellsSmall cytoplasmicMammalian cellsRRNA precursorTranscription factorsSmall nuclearU1 RNASplice junctionsRibonucleoproteinLa ribonucleoproteinsFurther characterizationProteinRelated diseasesCellsSplicingTRNARNA
1982
Deciphering the mysteries of RNA‐containing lupus antigens
Lerner E, Lerner M, Hardin J, Janeway C, Steitz J. Deciphering the mysteries of RNA‐containing lupus antigens. Arthritis & Rheumatism 1982, 25: 761-766. PMID: 6179522, DOI: 10.1002/art.1780250709.Peer-Reviewed Original Research
1981
Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.
Lerner MR, Andrews NC, Miller G, Steitz JA. Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 805-809. PMID: 6262773, PMCID: PMC319891, DOI: 10.1073/pnas.78.2.805.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusLupus erythematosusEBER-1EBER-2EBV DNAB lymphocytesEpstein-Barr virus genomeEpstein-Barr virusNormal B lymphocytesEcoRI J fragmentT lymphocytesErythematosusLa antigenLymphocytesPrimate cellsHerpesvirus atelesExhibit sequence homologyAntibodiesPotential relationshipVirus genomeCell transformationCellsUninfected mammalian cellsRNAMammalian cells