2004
Evidence for reassociation of RNA-binding proteins after cell lysis: Implications for the interpretation of immunoprecipitation analyses
Mili S, Steitz JA. Evidence for reassociation of RNA-binding proteins after cell lysis: Implications for the interpretation of immunoprecipitation analyses. RNA 2004, 10: 1692-1694. PMID: 15388877, PMCID: PMC1370654, DOI: 10.1261/rna.7151404.Peer-Reviewed Original Research
2000
HuR binding to cytoplasmic mRNA is perturbed by heat shock
Gallouzi I, Brennan C, Stenberg M, Swanson M, Eversole A, Maizels N, Steitz J. HuR binding to cytoplasmic mRNA is perturbed by heat shock. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 3073-3078. PMID: 10737787, PMCID: PMC16194, DOI: 10.1073/pnas.97.7.3073.Peer-Reviewed Original Research
1989
The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome.
Pinto AL, Steitz JA. The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 8742-8746. PMID: 2479028, PMCID: PMC298364, DOI: 10.1073/pnas.86.22.8742.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU5 small nuclear ribonucleoproteinHeLa cell nuclear extractsSmall nuclear ribonucleoprotein particleCell nuclear extractsAnti-trimethylguanosine antibodyNuclear ribonucleoprotein particleAffinity-purified spliceosomesSplicing proteinsMammalian proteinsYeast proteinsSnRNP complexMRNA splicingSplicing extractsRibonucleoprotein particleNuclear ribonucleoproteinMammalian analogueNuclear extractsSm classGradient fractionationSm epitopesProteinSpliceosomeProtein reactivePrp8
1984
The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies.
Pettersson I, Hinterberger M, Mimori T, Gottlieb E, Steitz JA. The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies. Journal Of Biological Chemistry 1984, 259: 5907-5914. PMID: 6232278, DOI: 10.1016/s0021-9258(18)91101-4.Peer-Reviewed Original ResearchConceptsSm small nuclear ribonucleoproteinsSmall nuclear ribonucleoproteinNuclear ribonucleoproteinProtein componentsAnti-Sm patient seraMultiple protein componentsSnRNP proteinsU6 RNAMammalian cellsDistinct polypeptidesAnti-Sm monoclonal antibodyRNA immunoprecipitationAnti-Sm autoantibodiesSnRNP polypeptidesProtein compositionSnRNP particlesProtein BMonoclonal antibodiesRibonucleoproteinIntriguing questionPolypeptideProteinBlot resultsMouse monoclonal antibodyEntire collection
1982
Deciphering the mysteries of RNA‐containing lupus antigens
Lerner E, Lerner M, Hardin J, Janeway C, Steitz J. Deciphering the mysteries of RNA‐containing lupus antigens. Arthritis & Rheumatism 1982, 25: 761-766. PMID: 6179522, DOI: 10.1002/art.1780250709.Peer-Reviewed Original Research
1981
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
Lerner EA, Lerner MR, Janeway CA, Steitz JA. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 2737-2741. PMID: 6789322, PMCID: PMC319432, DOI: 10.1073/pnas.78.5.2737.Peer-Reviewed Original ResearchConceptsAnti-Sm antibodiesMonoclonal anti-Sm antibodyAutoimmune diseasesMonoclonal antibodiesLupus erythematosus-like syndromeMRL/Mp-lpr/lprProduction of autoantibodiesPatient seraSpleen cellsImmunofluorescence techniqueAntibodiesSP 2/0Competition radioimmunoassayImmunoprecipitation patternsCell linesSm antigenAntigenDiseaseMiceCellular constituentsCellular componentsAutoantibodiesPatientsLupusSyndrome