2012
Tracking expression and subcellular localization of RNA and protein species using high-throughput single cell imaging flow cytometry
Borah S, Nichols LA, Hassman LM, Kedes DH, Steitz JA. Tracking expression and subcellular localization of RNA and protein species using high-throughput single cell imaging flow cytometry. RNA 2012, 18: 1573-1579. PMID: 22745225, PMCID: PMC3404377, DOI: 10.1261/rna.033126.112.Peer-Reviewed Original ResearchConceptsKaposi's sarcoma-associated herpesvirusSarcoma-associated herpesvirusSubcellular localizationProtein moleculesHigh-throughput approachPAN RNAProtein speciesNoncoding RNAsNuclear RNAProtein C1Imaging Flow CytometryFlow cytometryRNANuclear translocationHigh-throughput applicationsLytic phaseViral RNATranslocationExpressionLocalizationCellsHeterogeneous populationPABPC1CytometryMolecules
1988
A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells.
Steitz JA, Berg C, Hendrick JP, La Branche-Chabot H, Metspalu A, Rinke J, Yario T. A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells. Journal Of Cell Biology 1988, 106: 545-556. PMID: 3279045, PMCID: PMC2115095, DOI: 10.1083/jcb.106.3.545.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoantibodiesCell NucleolusCentrifugation, Density GradientElectrophoresis, Polyacrylamide GelFluorescent Antibody TechniqueFriend murine leukemia virusHeLa CellsHumansImmunoassayLeukemia, Erythroblastic, AcuteRibonucleoproteinsRibosomal ProteinsRibosomesRNA PrecursorsRNA, RibosomalRNA, Ribosomal, 5STumor Cells, CulturedConceptsRNA-protein complexesVivo pulse-chase experimentsRibosomal protein L5Pulse-chase experimentsProtein complexesProtein L5Mammalian cellsRNA moleculesMouse cellsLater stepsHeLa cellsProtein moietyRNPRibosomesNucleoliCellsComplexesAssemblyBiogenesisRRNAIndirect immunofluorescenceSubunitsRNAImmunofluorescence
1986
Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders.
Mimori T, Hardin JA, Steitz JA. Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders. Journal Of Biological Chemistry 1986, 261: 2274-2278. PMID: 3511059, DOI: 10.1016/s0021-9258(17)35929-x.Peer-Reviewed Original ResearchConceptsKu proteinNovel DNA-binding proteinAnti-Ku antibodiesDNA-binding proteinsHigh molecular weight nucleic acidsExtracts of cellsKu antigenDa subunitChromatinHeLa cellsScleroderma-polymyositis overlap syndromeBiochemical natureProteinWeight nucleic acidsLarge formNucleic acidsOverlap syndromeRheumatic disordersCertain patientsAntibodiesAntigenAutoantibodiesCellsImmunoaffinity column chromatographyPatients
1984
Autoantibodies to the U2 small nuclear ribonucleoprotein in a patient with scleroderma-polymyositis overlap syndrome.
Mimori T, Hinterberger M, Pettersson I, Steitz JA. Autoantibodies to the U2 small nuclear ribonucleoprotein in a patient with scleroderma-polymyositis overlap syndrome. Journal Of Biological Chemistry 1984, 259: 560-565. PMID: 6231285, DOI: 10.1016/s0021-9258(17)43698-2.Peer-Reviewed Original ResearchMeSH KeywordsAutoantibodiesFemaleFluorescent Antibody TechniqueHeLa CellsHumansImmunodiffusionMyositisRibonucleoproteinsRibonucleoproteins, Small NuclearScleroderma, SystemicConceptsU2 small nuclear ribonucleoproteinSmall nuclear ribonucleoproteinNuclear ribonucleoproteinU2 small nuclear RNASm small nuclear ribonucleoproteinsU6 small nuclear ribonucleoproteinProtein blot analysisSmall nuclear RNAScleroderma-polymyositis overlap syndromeU1 small nuclear ribonucleoproteinHeLa cell extractsRNA speciesNuclear RNAB proteinCell extractsProtein bandsBlot analysisOverlap syndromeRibonucleoproteinProteinAnti-Sm antibodiesU1InsectsRNAPolypeptide
1981
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
Lerner EA, Lerner MR, Janeway CA, Steitz JA. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 2737-2741. PMID: 6789322, PMCID: PMC319432, DOI: 10.1073/pnas.78.5.2737.Peer-Reviewed Original ResearchConceptsAnti-Sm antibodiesMonoclonal anti-Sm antibodyAutoimmune diseasesMonoclonal antibodiesLupus erythematosus-like syndromeMRL/Mp-lpr/lprProduction of autoantibodiesPatient seraSpleen cellsImmunofluorescence techniqueAntibodiesSP 2/0Competition radioimmunoassayImmunoprecipitation patternsCell linesSm antigenAntigenDiseaseMiceCellular constituentsCellular componentsAutoantibodiesPatientsLupusSyndrome