2002
The splicing of U12‐type introns can be a rate‐limiting step in gene expression
Patel AA, McCarthy M, Steitz JA. The splicing of U12‐type introns can be a rate‐limiting step in gene expression. The EMBO Journal 2002, 21: 3804-3815. PMID: 12110592, PMCID: PMC126102, DOI: 10.1093/emboj/cdf297.Peer-Reviewed Original ResearchConceptsU12-type intronsGene expressionDrosophila melanogaster S2 cellsProtein-coding genesU12-type spliceosomePost-transcriptional regulationHuman tissue culture cellsU2-type intronsMetazoan genomesTissue culture cellsS2 cellsU12-typeIntron removalIdentical mRNAIntronsFluorescent proteinQuantitative RT-PCR assayMinigene constructsCulture cellsRate-limiting stepSpliceosomeMRNAMinor classExpressionRT-PCR assays
1995
Site-specific crosslinking of 4-thiouridine-modified human tRNA(3Lys) to reverse transcriptase from human immunodeficiency virus type I.
Mishima Y, Steitz JA. Site-specific crosslinking of 4-thiouridine-modified human tRNA(3Lys) to reverse transcriptase from human immunodeficiency virus type I. The EMBO Journal 1995, 14: 2679-87. PMID: 7540137, PMCID: PMC398382, DOI: 10.1002/j.1460-2075.1995.tb07266.x.Peer-Reviewed Original Research
1992
Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing.
Wyatt JR, Sontheimer EJ, Steitz JA. Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing. Genes & Development 1992, 6: 2542-2553. PMID: 1340469, DOI: 10.1101/gad.6.12b.2542.Peer-Reviewed Original ResearchConceptsSplice siteMRNA splicingATP-dependent interactionHeLa nuclear extractsU5 snRNPMRNA substratesSplice site regionProtein factorsU5 snRNANucleotides upstreamU6 snRNAPre-mRNANuclear extractsSplicingSite regionLoop sequenceCross-link formationSnRNASplicing conditionsWatson-Crick complementarityCross-linking strategyU1Selective photoactivationSnRNPMammalianThe low-abundance U11 and U12 small nuclear ribonucleoproteins (snRNPs) interact to form a two-snRNP complex.
Wassarman KM, Steitz JA. The low-abundance U11 and U12 small nuclear ribonucleoproteins (snRNPs) interact to form a two-snRNP complex. Molecular And Cellular Biology 1992, 12: 1276-1285. PMID: 1372090, PMCID: PMC369560, DOI: 10.1128/mcb.12.3.1276.Peer-Reviewed Original Research
1991
An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
Baserga SJ, Yang XD, Steitz JA. An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs. The EMBO Journal 1991, 10: 2645-51. PMID: 1714385, PMCID: PMC452965, DOI: 10.1002/j.1460-2075.1991.tb07807.x.Peer-Reviewed Original ResearchConceptsBox CU3 snRNANucleolar small RNAsSite-specific mutationsShort nucleotide sequencesFibrillarin proteinSmall RNAsDeletion analysisCommon binding siteBox DNucleotide sequenceSnRNPsMajor familiesSnRNAU3 snRNPRNAInput RNAFibrillarinBinding sitesC sequencesBindingProteinSequenceAnti-fibrillarin autoantibodiesBiogenesisEAP, a highly conserved cellular protein associated with Epstein-Barr virus small RNAs (EBERs).
Toczyski DP, Steitz JA. EAP, a highly conserved cellular protein associated with Epstein-Barr virus small RNAs (EBERs). The EMBO Journal 1991, 10: 459-66. PMID: 1846807, PMCID: PMC452667, DOI: 10.1002/j.1460-2075.1991.tb07968.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceB-LymphocytesCell LineCell Transformation, ViralCloning, MolecularDNAHerpesvirus 4, HumanHumansMolecular Sequence DataPolymerase Chain ReactionProteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRibosomal ProteinsRNA, Small NuclearRNA, ViralRNA-Binding ProteinsSequence Homology, Nucleic Acid
1987
Human acidic ribosomal phosphoproteins P0, P1, and P2: analysis of cDNA clones, in vitro synthesis, and assembly.
Rich BE, Steitz JA. Human acidic ribosomal phosphoproteins P0, P1, and P2: analysis of cDNA clones, in vitro synthesis, and assembly. Molecular And Cellular Biology 1987, 7: 4065-4074. PMID: 3323886, PMCID: PMC368077, DOI: 10.1128/mcb.7.11.4065.Peer-Reviewed Original Research
1986
Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders.
Mimori T, Hardin JA, Steitz JA. Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders. Journal Of Biological Chemistry 1986, 261: 2274-2278. PMID: 3511059, DOI: 10.1016/s0021-9258(17)35929-x.Peer-Reviewed Original ResearchConceptsKu proteinNovel DNA-binding proteinAnti-Ku antibodiesDNA-binding proteinsHigh molecular weight nucleic acidsExtracts of cellsKu antigenDa subunitChromatinHeLa cellsScleroderma-polymyositis overlap syndromeBiochemical natureProteinWeight nucleic acidsLarge formNucleic acidsOverlap syndromeRheumatic disordersCertain patientsAntibodiesAntigenAutoantibodiesCellsImmunoaffinity column chromatographyPatients
1983
Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome
Wolin S, Steitz J. Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome. Cell 1983, 32: 735-744. PMID: 6187471, DOI: 10.1016/0092-8674(83)90059-4.Peer-Reviewed Original ResearchConceptsHuman genomeRo RNAsSecondary structure homologyRNA polymerase IIISmall cytoplasmic ribonucleoproteinsClass III genesGenomic clonesMammalian cellsPolymerase IIIRNA componentStructure homologySingle copyCytoplasmic ribonucleoproteinHY5 RNAMouse cellsHuman cellsHY1Cell extractsGenesRNAGenomeRNAsHY3CellsMY1
1978
Conservation of the primary structure at the 3′ end of 18S rRNA from eucaryotic cells
Hagenbüchle O, Santer M, Steitz J, Mans R. Conservation of the primary structure at the 3′ end of 18S rRNA from eucaryotic cells. Cell 1978, 13: 551-563. PMID: 77738, DOI: 10.1016/0092-8674(78)90328-8.Peer-Reviewed Original ResearchConceptsE. coli 16S rRNAE. coli ribosomesPurine-rich regionDNA sequencing methodsRRNA moleculesEucaryotic speciesStrong homologyCDNA transcriptsCDNA copyPolynucleotidyl transferasesRNA moleculesRRNA sequencesEucaryotic cellsEucaryotic mRNAsExtensive complementarityColi ribosomesRRNAPrimary structureEnzyme ATPSequencing methodsOligonucleotide primersAMV reverse transcriptaseTerminusReverse transcriptaseSequence