1984
The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies.
Pettersson I, Hinterberger M, Mimori T, Gottlieb E, Steitz JA. The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies. Journal Of Biological Chemistry 1984, 259: 5907-5914. PMID: 6232278, DOI: 10.1016/s0021-9258(18)91101-4.Peer-Reviewed Original ResearchConceptsSm small nuclear ribonucleoproteinsSmall nuclear ribonucleoproteinNuclear ribonucleoproteinProtein componentsAnti-Sm patient seraMultiple protein componentsSnRNP proteinsU6 RNAMammalian cellsDistinct polypeptidesAnti-Sm monoclonal antibodyRNA immunoprecipitationAnti-Sm autoantibodiesSnRNP polypeptidesProtein compositionSnRNP particlesProtein BMonoclonal antibodiesRibonucleoproteinIntriguing questionPolypeptideProteinBlot resultsMouse monoclonal antibodyEntire collectionThe Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs.
Wolin SL, Steitz JA. The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 1996-2000. PMID: 6201849, PMCID: PMC345423, DOI: 10.1073/pnas.81.7.1996.Peer-Reviewed Original ResearchConceptsRibonucleoprotein particleHeLa cellsSmall cytoplasmic RNARibonuclease protection experimentsSmall ribonucleoprotein particlesSmall cytoplasmic ribonucleoproteinsHuman HeLa cellsCytoplasmic ribonucleoproteinMammalian speciesCytoplasmic RNARNA sequencesMajor proteinsRo RNAsProtection experimentsRo proteinPossible functionsBase pairsIdentical base pairsRNAProteinSystemic lupus erythematosusAntigenic proteinsAntigenic polypeptidesRo antibodiesLupus erythematosus
1981
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
Lerner EA, Lerner MR, Janeway CA, Steitz JA. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 2737-2741. PMID: 6789322, PMCID: PMC319432, DOI: 10.1073/pnas.78.5.2737.Peer-Reviewed Original ResearchConceptsAnti-Sm antibodiesMonoclonal anti-Sm antibodyAutoimmune diseasesMonoclonal antibodiesLupus erythematosus-like syndromeMRL/Mp-lpr/lprProduction of autoantibodiesPatient seraSpleen cellsImmunofluorescence techniqueAntibodiesSP 2/0Competition radioimmunoassayImmunoprecipitation patternsCell linesSm antigenAntigenDiseaseMiceCellular constituentsCellular componentsAutoantibodiesPatientsLupusSyndromeSequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing
Mount S, Steitz J. Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing. Nucleic Acids Research 1981, 9: 6351-6368. PMID: 6172778, PMCID: PMC327608, DOI: 10.1093/nar/9.23.6351.Peer-Reviewed Original ResearchConceptsU1 RNAU1 sequenceCultured Drosophila melanogaster cellsDrosophila U1 RNAU1 RNA sequenceDrosophila melanogaster cellsHuman U1 RNASecondary structure modelDNA sequence determinationDonor splice junctionGenomic clonesDrosophila melanogasterSplicing reactionRNA moleculesU1 snRNPsConsensus sequenceSplice junctionsRNA sequencesNucleotides 3Sequence determinationSecondary structureRNASplicingMolecular interactionsSequence