2008
Primary microRNA transcript retention at sites of transcription leads to enhanced microRNA production
Pawlicki JM, Steitz JA. Primary microRNA transcript retention at sites of transcription leads to enhanced microRNA production. Journal Of Cell Biology 2008, 182: 61-76. PMID: 18625843, PMCID: PMC2447899, DOI: 10.1083/jcb.200803111.Peer-Reviewed Original ResearchMeSH KeywordsChromatinExonsHeLa CellsHumansIntronsMicroRNAsMRNA Cleavage and Polyadenylation FactorsNuclear ProteinsPolyadenylationRegulatory Sequences, Nucleic AcidRibonucleoproteinsRNA Polymerase IIIRNA Processing, Post-TranscriptionalRNA TransportRNA, MessengerRNA, ViralSequence DeletionSerine-Arginine Splicing FactorsSubcellular FractionsTranscription, GeneticConceptsPri-miRNAsTranscription sitesEndogenous pri-miRNAsPrimary miRNA transcriptsPri-miRNA processingSplicing factor SC35Viral RNA elementsHigh nuclear levelsMiRNA biogenesisMiRNA transcriptionMiRNA transcriptsNuclear stepsPrecursor miRNAsNuclear fociFactor SC35MicroRNA productionRNA elementsGene expressionTranscription leadNuclear fractionNuclear levelsTranscriptionMiRNAsProcessing signalsBiogenesis
1989
Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III.
Gottlieb E, Steitz JA. Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III. The EMBO Journal 1989, 8: 851-861. PMID: 2470590, PMCID: PMC400884, DOI: 10.1002/j.1460-2075.1989.tb03446.x.Peer-Reviewed Original ResearchConceptsRNA polymerase III transcriptionPolymerase III transcriptionRNA polymerase IIITranscription complexPolymerase IIILa proteinTranscription termination factorFull-length transcriptsTranscription terminationTermination factorRNA productsTranscription intermediatesTranscriptsTranscriptionProteinComplexesPolymeraseRegulatorAbsenceThe RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro.
Gottlieb E, Steitz JA. The RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro. The EMBO Journal 1989, 8: 841-850. PMID: 2498086, PMCID: PMC400883, DOI: 10.1002/j.1460-2075.1989.tb03445.x.Peer-Reviewed Original ResearchConceptsTranscription levelsNascent RNA polymerase III transcriptsRNA polymerase III transcriptionAbundant nuclear phosphoproteinRNA polymerase III transcriptsPolymerase III transcriptionPolymerase III transcriptsClass III genesHeLa cell extractsAction of LaTranscript lengthTermination signalNuclear phosphoproteinUridylate residuesLa proteinTranscription activityAutoantigen LaCell extractsTranscriptsRNAAbsence of LAMouse monoclonal antibodyTranscriptionPhosphoproteinGenes
1983
Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome
Wolin S, Steitz J. Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome. Cell 1983, 32: 735-744. PMID: 6187471, DOI: 10.1016/0092-8674(83)90059-4.Peer-Reviewed Original ResearchConceptsHuman genomeRo RNAsSecondary structure homologyRNA polymerase IIISmall cytoplasmic ribonucleoproteinsClass III genesGenomic clonesMammalian cellsPolymerase IIIRNA componentStructure homologySingle copyCytoplasmic ribonucleoproteinHY5 RNAMouse cellsHuman cellsHY1Cell extractsGenesRNAGenomeRNAsHY3CellsMY1Small ribonucleoproteins from eukaryotes: structures and roles in RNA biogenesis.
Steitz JA, Wolin SL, Rinke J, Pettersson I, Mount SM, Lerner EA, Hinterberger M, Gottlieb E. Small ribonucleoproteins from eukaryotes: structures and roles in RNA biogenesis. Cold Spring Harbor Symposia On Quantitative Biology 1983, 47 Pt 2: 893-900. PMID: 6222869, DOI: 10.1101/sqb.1983.047.01.103.Peer-Reviewed Original ResearchStructure and Function of Small Ribonucleoproteins from Eukaryotic Cells
Steitz JA, Berg C, Gottlieb E, Hardin JA, Hashimoto C, Hendrick JP, Hinterberger M, Krikeles M, Lerner MR, Mount SM, Pettersson I, Rinke J, Rosa M, Wolin S. Structure and Function of Small Ribonucleoproteins from Eukaryotic Cells. 1983, 12: 309-317. PMID: 7166547, DOI: 10.1016/b978-0-12-501650-6.50023-7.Peer-Reviewed Original ResearchConceptsSmall ribonucleoproteinLa proteinRNA polymerase III transcription factorSmall RNA-protein complexesDrosophila U1 RNAU1 small nuclearRNA-protein complexesHnRNA splicingEukaryotic cellsSmall cytoplasmicMammalian cellsRRNA precursorTranscription factorsSmall nuclearU1 RNASplice junctionsRibonucleoproteinLa ribonucleoproteinsFurther characterizationProteinRelated diseasesCellsSplicingTRNARNA