2004
Premature termination codons do not affect the rate of splicing of neighboring introns
Lytle JR, Steitz JA. Premature termination codons do not affect the rate of splicing of neighboring introns. RNA 2004, 10: 657-668. PMID: 15037775, PMCID: PMC1370556, DOI: 10.1261/rna.5241404.Peer-Reviewed Original Research
1997
AU-rich elements target small nuclear RNAs as well as mRNAs for rapid degradation
Fan X, Myer V, Steitz J. AU-rich elements target small nuclear RNAs as well as mRNAs for rapid degradation. Genes & Development 1997, 11: 2557-2568. PMID: 9334320, PMCID: PMC316563, DOI: 10.1101/gad.11.19.2557.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, SurfaceBase SequenceELAV ProteinsELAV-Like Protein 1Gene Expression RegulationGenes, ReporterGlobinsHerpesvirus 2, SaimiriineMolecular Sequence DataMutationRepetitive Sequences, Nucleic AcidRibonucleasesRNA, MessengerRNA, Small NuclearRNA, ViralRNA-Binding ProteinsTranscription, GeneticTransfectionConceptsAU-rich elementsMRNA degradation machinerySmall nuclear RNAHSUR 1Host RNA moleculesDegradation machineryMammalian mRNAsNuclear RNARNA moleculesMutational analysisSequence requirementsTarget RNAHuR proteinOngoing translationRNA 1MRNARapid degradationRNASimilar mechanismDegradation activityDeadenylationSnRNAMachineryProteinDegradation
1989
[31] Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing
Parker K, Steitz J. [31] Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing. Methods In Enzymology 1989, 180: 454-468. PMID: 2482428, DOI: 10.1016/0076-6879(89)80117-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesCarrier ProteinsElectrophoresis, Polyacrylamide GelEndoribonucleasesHumansIndicators and ReagentsKineticsMiceMicrococcal NucleaseNucleotide MappingRibonuclease HRibonuclease T1RibonucleasesRibonucleoproteinsRNARNA PrecursorsRNA SplicingRNA, Ribosomal, 28SRNA-Binding ProteinsTranscription, Genetic
1985
Transfer RNA is an essential component of the ubiquitin- and ATP-dependent proteolytic system.
Ciechanover A, Wolin SL, Steitz JA, Lodish HF. Transfer RNA is an essential component of the ubiquitin- and ATP-dependent proteolytic system. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 1341-1345. PMID: 2983338, PMCID: PMC397256, DOI: 10.1073/pnas.82.5.1341.Peer-Reviewed Original ResearchConceptsATP-dependent proteolytic systemProteolytic systemATP-dependent degradationATP-requiring reactionATP-dependent pathwayProteolytic activityProgression of cellsProtein substratesTransfer RNAGene activationMammalian cellsUncharged tRNAReticulocyte extractsProtein degradationTRNA fractionReticulocyte systemMouse cellsCell cycleUbiquitinEssential componentRNAGel electrophoresisTRNAConjugated proteinsAutoimmune sera
1980
The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli
Bram R, Young R, Steitz J. The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli. Cell 1980, 19: 393-401. PMID: 6153577, DOI: 10.1016/0092-8674(80)90513-9.Peer-Reviewed Original Research
1977
Characterization of two mRNA · rRNA complexes implicated in the initiation of protein biosynthesis
Steitz J, Steege D. Characterization of two mRNA · rRNA complexes implicated in the initiation of protein biosynthesis. Journal Of Molecular Biology 1977, 114: 545-558. PMID: 335077, DOI: 10.1016/0022-2836(77)90177-2.Peer-Reviewed Original ResearchConceptsProtein biosynthesisEscherichia coli 16 S ribosomal RNAS ribosomal RNABase pair regionNuclease digestion studiesFragment complexAssignment of residuesRibosomal RNARRNA complexLambda PRBacteriophage lambdaMolecular understandingInitiation siteInitiation eventsStrong experimental supportMessenger RNABiosynthesisThermal denaturation studiesDenaturation studiesRNAPR transcriptsPR regionDigestion studiesMRNAComplexes
1975
The 3′ terminal oligonucleotide of E.coli 16S ribosomal RNA: the sequence in both wild-type and RNase III cells is complementary to the polypurine tracts common to mRNA initiator regions
Sprague K, Steitz J. The 3′ terminal oligonucleotide of E.coli 16S ribosomal RNA: the sequence in both wild-type and RNase III cells is complementary to the polypurine tracts common to mRNA initiator regions. Nucleic Acids Research 1975, 2: 787-798. PMID: 167351, PMCID: PMC343466, DOI: 10.1093/nar/2.6.787.Peer-Reviewed Original Research
1974
Nucleotide sequences of the 5′ and 3′ termini of bacteriophage T7 early messenger RNAs synthesized in vivo: Evidence for sequence specificity in RNA processing
Kramer R, Rosenberg M, Steitz J. Nucleotide sequences of the 5′ and 3′ termini of bacteriophage T7 early messenger RNAs synthesized in vivo: Evidence for sequence specificity in RNA processing. Journal Of Molecular Biology 1974, 89: 767-776. PMID: 4449132, DOI: 10.1016/0022-2836(74)90051-5.Peer-Reviewed Original ResearchConceptsEarly regionMessenger RNAAdenylic acid residuesEarly messenger RNATwo-dimensional electrophoretic techniquesLarger precursor moleculeMonocistronic messengerRNA processingGene 0.3RNA speciesNucleotide sequenceSequence specificityAcid residuesMessenger speciesSequence analysisInitiator RNASequence heterogeneityRNAEarly RNATerminusT7 Early messenger RNAs are the direct products of ribonuclease III cleavage
Rosenberg M, Kramer R, Steitz J. T7 Early messenger RNAs are the direct products of ribonuclease III cleavage. Journal Of Molecular Biology 1974, 89: 777-782. PMID: 4615167, DOI: 10.1016/0022-2836(74)90052-7.Peer-Reviewed Original ResearchDirect physical evidence for secondary structure in an isolated fragment of R17 bacteriophage mRNA
Gralla J, Steitz J, Crothers D. Direct physical evidence for secondary structure in an isolated fragment of R17 bacteriophage mRNA. Nature 1974, 248: 204-208. PMID: 4819414, DOI: 10.1038/248204a0.Peer-Reviewed Original Research
1973
Correction for non-uniform phosphate labeling of E. coli and phage RNAs synthesized in vivo
Kramer R, Steitz J. Correction for non-uniform phosphate labeling of E. coli and phage RNAs synthesized in vivo. Biochemical And Biophysical Research Communications 1973, 54: 1198-1202. PMID: 4584881, DOI: 10.1016/0006-291x(73)90819-x.Peer-Reviewed Original ResearchDiscriminatory Ribosome Rebinding of Isolated Regions of Protein Synthesis Initiation from the Ribonucleic Acid of Bacteriophage R17
Steitz J. Discriminatory Ribosome Rebinding of Isolated Regions of Protein Synthesis Initiation from the Ribonucleic Acid of Bacteriophage R17. Proceedings Of The National Academy Of Sciences Of The United States Of America 1973, 70: 2605-2609. PMID: 4582190, PMCID: PMC427065, DOI: 10.1073/pnas.70.9.2605.Peer-Reviewed Original ResearchConceptsR17 RNARibosome-binding siteProtein synthesis initiationEscherichia coli ribosomesPolypeptide chain initiationCoat protein regionRibosome bindingBacterial ribosomeRNA moleculesInitiator regionRibosome protectionSynthesis initiationMRNA moleculesInitiator codonColi ribosomesRibosomesNative RNABacteriophage R17Bacillus stearothermophilusRNAMessenger RNARibonucleic acidR17Replicase regionFragmentsSpecific recognition of non-initiator regions in RNA bacteriophage messengers by ribosomes of Bacillus stearothermophilus
Steitz J. Specific recognition of non-initiator regions in RNA bacteriophage messengers by ribosomes of Bacillus stearothermophilus. Journal Of Molecular Biology 1973, 73: 1-16. PMID: 4570382, DOI: 10.1016/0022-2836(73)90155-1.Peer-Reviewed Original ResearchBacillusBase SequenceBinding SitesCentrifugation, Density GradientChromatography, Thin LayerColiphagesDipeptidesEscherichia coliGenetic CodeNucleotidesPeptide Chain Initiation, TranslationalPeptide Initiation FactorsRibonucleasesRibosomesRNA, MessengerRNA, TransferRNA, ViralSpecies SpecificityTemperatureViral Proteins
1972
Oligonucleotide Sequence of Replicase Initiation Site in Qβ RNA
STEITZ J. Oligonucleotide Sequence of Replicase Initiation Site in Qβ RNA. Nature 1972, 236: 71-75. PMID: 4502455, DOI: 10.1038/newbio236071a0.Peer-Reviewed Original ResearchConceptsReplicase geneQβ RNAMajor coat proteinN-terminal sequenceCoat cistronTermination signalReplicase cistronNucleotide sequenceCoat proteinRNA genomeInitiator codonInitiation siteQβ replicaseGenesProteinBacteriophage QβCistronUGA suppressorRNAVirus particlesSequenceOligonucleotide sequencesProtein7GenomeReplicase
1969
Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNA
STEITZ J. Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNA. Nature 1969, 224: 957-964. PMID: 5360547, DOI: 10.1038/224957a0.Peer-Reviewed Original Research
1968
A slowly sedimenting, infective form of bacteriophage R17
Steitz J. A slowly sedimenting, infective form of bacteriophage R17. Journal Of Molecular Biology 1968, 33: 947-951. PMID: 5700427, DOI: 10.1016/0022-2836(68)90330-6.Peer-Reviewed Original Research