A general two-metal-ion mechanism for catalytic RNA.
Steitz TA, Steitz JA. A general two-metal-ion mechanism for catalytic RNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 6498-6502. PMID: 8341661, PMCID: PMC46959, DOI: 10.1073/pnas.90.14.6498.Peer-Reviewed Original ResearchConceptsMetal ionsTwo-metal-ion mechanismCatalytic metal ionPhosphoryl transfer reactionsChemical catalysisLewis acidTransfer reactionsReaction pathwaysTransition stateProtein enzymesCatalytic siteSugar hydroxylsIonsGroup I self-splicing intronCatalytic RNAReactionSelf-splicing intronsP hydrolysisCatalysisBinding sitesOxyanionsHydroxylSpecific binding sitesHydrolysisAcid