The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
Mount S, Pettersson I, Hinterberger M, Karmas A, Steitz J. The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro. Cell 1983, 33: 509-518. PMID: 6190573, DOI: 10.1016/0092-8674(83)90432-4.Peer-Reviewed Original ResearchConceptsRNA-protein complexesSplice siteU1 snRNPGlobin RNAPartial proteinase K digestionU1 snRNPsMouse beta-globin geneBacteriophage T7 RNA polymeraseEucaryotic cell nucleusMessenger RNA splicingT7 RNA polymeraseRecombinant DNA clonesBeta-globin geneT1 ribonuclease digestionSplice site sequencesSmall intronsSnRNP proteinsRNA splicingSnRNP complexDNA clonesRNA polymeraseSnRNPSite sequenceRibonuclease digestionProteinase K digestion