2019
Effects of Endotoxin on Type 3 Inositol 1,4,5‐Trisphosphate Receptor in Human Cholangiocytes
Franca A, Filho A, Guerra MT, Weerachayaphorn J, dos Santos M, Njei B, Robert M, Lima C, Vidigal P, Banales JM, Ananthanarayanan M, Leite MF, Nathanson MH. Effects of Endotoxin on Type 3 Inositol 1,4,5‐Trisphosphate Receptor in Human Cholangiocytes. Hepatology 2019, 69: 817-830. PMID: 30141207, PMCID: PMC6351171, DOI: 10.1002/hep.30228.Peer-Reviewed Original ResearchConceptsToll-like receptor 4Alcoholic hepatitisEffect of endotoxinBile duct cellsNF-κBInhibition of TLR4Human cholangiocytesStimulation of TLR4Duct cellsSevere alcoholic hepatitisCholestasis of sepsisForms of cholestasisNF-κB subunitsP65/p50Trisphosphate receptorReceptor 4Clinical conditionsBicarbonate secretionHepatocellular changesITPR3 expressionCholestasisType 3 inositolLPS receptorAgonist stimulusSepsis
2017
Type 2 inositol trisphosphate receptor gene expression in hepatocytes is regulated by cyclic AMP
Kruglov E, Ananthanarayanan M, Sousa P, Weerachayaphorn J, Guerra MT, Nathanson MH. Type 2 inositol trisphosphate receptor gene expression in hepatocytes is regulated by cyclic AMP. Biochemical And Biophysical Research Communications 2017, 486: 659-664. PMID: 28327356, PMCID: PMC5421629, DOI: 10.1016/j.bbrc.2017.03.086.Peer-Reviewed Original ResearchMeSH KeywordsAdenylyl CyclasesAnimalsBinding SitesColforsinCREB-Binding ProteinCyclic AMPDactinomycinFastingGene Expression RegulationHep G2 CellsHepatocytesHumansInositol 1,4,5-Trisphosphate ReceptorsMaleMutationPrimary Cell CulturePromoter Regions, GeneticProtein BindingRatsRats, Sprague-DawleyResponse ElementsRNA, MessengerSignal TransductionThionucleotidesConceptsPost-translational modificationsRecruitment of CREBAdenylyl cyclase 6Transcriptional regulationType 2 inositolGene expressionPromoter activityTrisphosphate receptorCyclase 6CRE elementTreatment of hepatocytesReceptor gene expressionAC isoformsCREBHormonal regulationProtein levelsIntracellular CaD. AnalysisPromoterRelease channelExpressionCyclic AMPIP3R2RegulationRat hepatocytes
2016
Effects of andrographolide on intrahepatic cholestasis induced by alpha-naphthylisothiocyanate in rats
Khamphaya T, Chansela P, Piyachaturawat P, Suksamrarn A, Nathanson MH, Weerachayaphorn J. Effects of andrographolide on intrahepatic cholestasis induced by alpha-naphthylisothiocyanate in rats. European Journal Of Pharmacology 2016, 789: 254-264. PMID: 27475677, PMCID: PMC10804355, DOI: 10.1016/j.ejphar.2016.07.032.Peer-Reviewed Original ResearchMeSH Keywords1-NaphthylisothiocyanateAnimalsBile DuctsCell ProliferationCholestasis, IntrahepaticCyclin D1DiterpenesGene Expression RegulationHepatic Stellate CellsLiverMaleNF-kappa BRatsConceptsCholestatic liver diseaseLiver diseaseIntrahepatic cholestasisLiver injuryProtective effectHepatic stellate cell activationAcute intrahepatic cholestasisAlpha-smooth muscle actinCholestatic liver injuryBile duct proliferationSerum alanine aminotransferaseNF-κB expressionSingle intraperitoneal injectionEffects of andrographolidePromising therapeutic optionEffective therapeutic approachPotent protective propertiesNuclear factor kappaStellate cell activationANIT injectionDuct proliferationTherapeutic optionsHepatoprotective effectPeriductular fibrosisAlternative therapies
2007
Identification of transport pathways for citric acid cycle intermediates in the human colon carcinoma cell line, Caco-2
Weerachayaphorn J, Pajor AM. Identification of transport pathways for citric acid cycle intermediates in the human colon carcinoma cell line, Caco-2. Biochimica Et Biophysica Acta 2007, 1778: 1051-1059. PMID: 18194662, PMCID: PMC2323910, DOI: 10.1016/j.bbamem.2007.12.013.Peer-Reviewed Original ResearchBiological TransportCaco-2 CellsCitratesCitric Acid CycleColonic NeoplasmsDicarboxylic Acid TransportersEpithelial CellsExtracellular SpaceGene Expression RegulationHumansIntracellular SpaceKineticsModels, BiologicalOrganic Anion TransportersOrganic Anion Transporters, Sodium-DependentPlasticsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodiumSubstrate SpecificitySuccinatesSymportersTime FactorsTransfection