2022
Molecular determinants of peri‐apical targeting of inositol 1,4,5‐trisphosphate receptor type 3 in cholangiocytes
Rodrigues MA, Gomes DA, Fiorotto R, Guerra MT, Weerachayaphorn J, Bo T, Sessa WC, Strazzabosco M, Nathanson MH. Molecular determinants of peri‐apical targeting of inositol 1,4,5‐trisphosphate receptor type 3 in cholangiocytes. Hepatology Communications 2022, 6: 2748-2764. PMID: 35852334, PMCID: PMC9512452, DOI: 10.1002/hep4.2042.Peer-Reviewed Original ResearchConceptsLipid raftsCaveolin-1Intact lipid raftsType 3 inositol trisphosphate receptorApical regionC-terminal amino acidsTrisphosphate receptor type 3Madin-Darby canine kidney cellsCanine kidney cellsFluorescence microscopy techniquesInositol trisphosphate receptorApical localizationTrisphosphate receptorHeavy chain 9Molecular determinantsChemical disruptionAmino acidsITPR3RaftsKidney cellsIntracellular CaFinal common eventReceptor type 3Release channelMYH9
2018
Nonalcoholic fatty liver disease impairs expression of the type II inositol 1,4,5‐trisphosphate receptor
Khamphaya T, Chukijrungroat N, Saengsirisuwan V, Mitchell‐Richards K, Robert ME, Mennone A, Ananthanarayanan M, Nathanson MH, Weerachayaphorn J. Nonalcoholic fatty liver disease impairs expression of the type II inositol 1,4,5‐trisphosphate receptor. Hepatology 2018, 67: 560-574. PMID: 29023819, PMCID: PMC5893412, DOI: 10.1002/hep.29588.Peer-Reviewed Original ResearchConceptsNonalcoholic fatty liver diseaseImpaired liver regenerationNonalcoholic steatohepatitisLiver regenerationHuh7 cellsLiver diseaseEffect of NAFLDPrevalent liver diseaseFatty liver diseaseC-JunHigh-fructose dietLiver biopsy specimensCell proliferationCalcium signalingHepG2 cellsLiver of ratsCell nuclear antigenCalcium release channelSimple steatosisLiver biopsyFatty liverTrisphosphate receptorBiopsy specimensRat modelType II inositol
2015
Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes
Weerachayaphorn J, Amaya MJ, Spirli C, Chansela P, Mitchell-Richards KA, Ananthanarayanan M, Nathanson MH. Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes. Gastroenterology 2015, 149: 211-222.e10. PMID: 25796361, PMCID: PMC4478166, DOI: 10.1053/j.gastro.2015.03.014.Peer-Reviewed Original ResearchConceptsBile ductBile duct unitsCholestatic disordersOxidative stressCalcium signalingNuclear factorMouse cholangiocytesDuct unitsReduced calcium signalingIntrahepatic bile ductsLevels of Nrf2Cholangiocyte cellsKnockdown of Nrf2Activation of Nrf2Intracellular calcium release channelsTranscription factor Nrf2Binding of Nrf2Calcium release channelPolymerase chain reaction analysisBiliary diseaseTrisphosphate receptorControl ratsLiver disordersBicarbonate secretionChain reaction analysis
2013
Deleterious effect of oltipraz on extrahepatic cholestasis in bile duct-ligated mice
Weerachayaphorn J, Luo Y, Mennone A, Soroka CJ, Harry K, Boyer JL. Deleterious effect of oltipraz on extrahepatic cholestasis in bile duct-ligated mice. Journal Of Hepatology 2013, 60: 160-166. PMID: 23978715, PMCID: PMC4054607, DOI: 10.1016/j.jhep.2013.08.015.Peer-Reviewed Original ResearchConceptsBile duct ligationBDL miceLiver injuryControl miceBile duct-ligated miceBile flow rateBile duct obstructionHepato-protective effectsSmooth muscle actin expressionLiver function markersSevere liver damageBile acid-independent flowHigher bile flowMatrix metalloproteinases-9Hepatic stellate cellsCancer preventive agentsMuscle actin expressionPromising cancer-preventive agentBiliary pressureSerum aminotransferasesLiver histologyBDL groupPhase II detoxificationPortal fibroblastsProfibrogenic genes
2012
Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine
Weerachayaphorn J, Mennone A, Soroka CJ, Harry K, Hagey LR, Kensler TW, Boyer JL. Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine. AJP Gastrointestinal And Liver Physiology 2012, 302: g925-g936. PMID: 22345550, PMCID: PMC3362073, DOI: 10.1152/ajpgi.00263.2011.Peer-Reviewed Original ResearchConceptsBile duct ligationBile acid homeostasisBile acid synthesisBile acidsLiver injuryAcid homeostasisMRNA expressionWild-type control miceBile acid transporter expressionCYP3A11 mRNA expressionBile salt export pumpBiliary bile acidsDeletion of Nrf2Bile acid reabsorptionRole of Nrf2Factor 2Bile acid hydroxylationPregnane X receptorBDL miceExpression of regulatorsControl miceDuct ligationNrf2 resultsBile secretionHepatobiliary transporters
2010
Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression
Xu S, Weerachayaphorn J, Cai SY, Soroka CJ, Boyer JL. Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression. AJP Gastrointestinal And Liver Physiology 2010, 299: g126-g135. PMID: 20395535, PMCID: PMC2904108, DOI: 10.1152/ajpgi.00522.2010.Peer-Reviewed Original ResearchMeSH Keywords5' Flanking RegionAnimalsAryl Hydrocarbon Receptor Nuclear TranslocatorBase SequenceBasic Helix-Loop-Helix Transcription FactorsBinding SitesButylaminesChromatin ImmunoprecipitationDose-Response Relationship, DrugElectrophoretic Mobility Shift AssayGenes, ReporterHep G2 CellsHepatocytesHumansMethylcholanthreneMiceMolecular Sequence DataMultidrug Resistance-Associated ProteinsMutationNF-E2-Related Factor 2Polychlorinated DibenzodioxinsPromoter Regions, GeneticPyrazinesReceptors, Aryl HydrocarbonResponse ElementsRNA InterferenceRNA, MessengerThionesThiophenesTime FactorsTransfectionConceptsMRP4 expressionAryl hydrocarbon receptorNF-E2Renal proximal tubule epitheliumCholestatic liver injuryHydrocarbon receptorXenobiotic response elementProximal tubule epitheliumMultidrug resistance protein 4Factor 2Bile salt conjugatesNuclear receptor agonistsLiver injuryPromoter activityReceptor agonistTherapeutic benefitAnimal modelsNrf2 activatorsTubule epitheliumHuman MRP4Conjugated steroidsNuclear factorAdaptive upregulationOxidative stressLuciferase reporter