2024
Composition Heterogeneity of Metal Ions Bound at the Oxygen-Evolving Center of Photosystem II in Living Cells
Wang J. Composition Heterogeneity of Metal Ions Bound at the Oxygen-Evolving Center of Photosystem II in Living Cells. Biochemistry 2024, 63: 1963-1968. PMID: 39037205, DOI: 10.1021/acs.biochem.4c00261.Peer-Reviewed Original ResearchConceptsX-ray free-electron lasersStructure of photosystem IIOxygen-evolving centerPhotosystem IICrystal structure of photosystem IIFree-electron laserPhotosystem II dimersPhotosystem ILight-harvesting complexesCryo-electron tomographyMetal ionsPSII samplesCryo-electron microscopyCryo-ETDimeric core complexesCryo-EMMetal ion cofactorsLiving cellsCrystal structureCryo-electronCryo-EM structureMetal-ion occupancyIon occupancySpectroscopic interpretationAsymmetric environment
2017
Insights into Photosystem II from Isomorphous Difference Fourier Maps of Femtosecond X‑ray Diffraction Data and Quantum Mechanics/Molecular Mechanics Structural Models
Wang J, Askerka M, Brudvig GW, Batista VS. Insights into Photosystem II from Isomorphous Difference Fourier Maps of Femtosecond X‑ray Diffraction Data and Quantum Mechanics/Molecular Mechanics Structural Models. ACS Energy Letters 2017, 2: 397-407. PMID: 28217747, PMCID: PMC5307371, DOI: 10.1021/acsenergylett.6b00626.Peer-Reviewed Original ResearchX-ray free-electron lasersFree-electron laserLight oxygen atomsHigh-resolution spectroscopyFemtosecond X-ray crystallographyPhotosystem IIQuantum mechanics/molecular mechanics techniquesBiomimetic photocatalytic systemsFunction of PSIIDifference Fourier mapsDiffraction dataElectron laserConventional X-ray diffraction methodXFEL dataHeavy metal centersS-state transitionsStructure-function determinationX-ray diffraction dataFourier mapsRadiation damageMolecular mechanics techniquesX-ray diffraction methodsOutstanding challengesOxygen atomsDiffraction methods
2016
Destruction‐and‐diffraction by X‐ray free‐electron laser
Wang J. Destruction‐and‐diffraction by X‐ray free‐electron laser. Protein Science 2016, 25: 1585-1592. PMID: 27262052, PMCID: PMC5338243, DOI: 10.1002/pro.2959.Peer-Reviewed Original ResearchConceptsX-ray free-electron lasersFree-electron laserSingle femtosecond pulseAtomic scattering factorsFemtosecond pulsesRecent theoretical studiesScattering factorsSuch pulsesElectronic processesDifferent atomsCrystallographic data collectionRadiation damageSingle pulseX-rayPulsesLaserSuch crystalsTheoretical studyCrystalsDiffractionMacromolecular crystalsFemtosecondExperimental evidenceAtomsRadiation