2012
Structural and mechanistic insights into guanylylation of RNA-splicing ligase RtcB joining RNA between 3′-terminal phosphate and 5′-OH
Englert M, Xia S, Okada C, Nakamura A, Tanavde V, Yao M, Eom SH, Konigsberg WH, Söll D, Wang J. Structural and mechanistic insights into guanylylation of RNA-splicing ligase RtcB joining RNA between 3′-terminal phosphate and 5′-OH. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 15235-15240. PMID: 22949672, PMCID: PMC3458315, DOI: 10.1073/pnas.1213795109.Peer-Reviewed Original ResearchConceptsRNA substratesRNA strandRNA phosphate backboneRNA endExtensive mutagenesisSecond RNA substrateKey residuesLigation pathwayBiochemical experimentsOverall ligationRNA ligaseGuanylylationRtcBMechanistic insightsGTP/Critical rolePhosphate backboneGMPActive siteCyclic phosphateDependent reactionDetailed insightStrandsLigaseMutagenesis
2003
The structural basis of cysteine aminoacylation of tRNAPro by prolyl-tRNA synthetases
Kamtekar S, Kennedy WD, Wang J, Stathopoulos C, Söll D, Steitz TA. The structural basis of cysteine aminoacylation of tRNAPro by prolyl-tRNA synthetases. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 1673-1678. PMID: 12578991, PMCID: PMC149891, DOI: 10.1073/pnas.0437911100.Peer-Reviewed Original ResearchConceptsLigand-free conformationCognate amino acidAmino acidsRecent biochemical experimentsProlyl-tRNA synthetasesCysteinyl-tRNA synthetaseProlyl-tRNA synthetaseActive site pocketMethanocaldococcus jannaschiiMethanopyrus kandleriMethanothermobacter thermautotrophicusCognate tRNAEssential enzymeApo formStructural basisBiochemical experimentsAminoacyl-adenylate analoguesHomology modelingConformational changesProtein synthesisTRNAProRSAdenylate complexNanomolar affinitySynthetase