2020
Comprehensive characterization of hepatocyte-derived extracellular vesicles identifies direct miRNA-based regulation of hepatic stellate cells and DAMP-based hepatic macrophage IL-1β and IL-17 upregulation in alcoholic hepatitis mice
Eguchi A, Yan R, Pan S, Wu R, Kim J, Chen Y, Ansong C, Smith R, Tempaku M, Ohno-Machado L, Takei Y, Feldstein A, Tsukamoto H. Comprehensive characterization of hepatocyte-derived extracellular vesicles identifies direct miRNA-based regulation of hepatic stellate cells and DAMP-based hepatic macrophage IL-1β and IL-17 upregulation in alcoholic hepatitis mice. Journal Of Molecular Medicine 2020, 98: 1021-1034. PMID: 32556367, PMCID: PMC7810220, DOI: 10.1007/s00109-020-01926-7.Peer-Reviewed Original ResearchConceptsHepatic stellate cellsAlcoholic liver diseaseAlcoholic hepatitisAH miceIL-1βHepatic macrophagesStellate cellsExtracellular vesiclesPrimary hepatic stellate cellsIL-17 upregulationIL-17 productionUpregulated IL-1βHepatocyte-derived extracellular vesiclesNovel murine modelTLR9-dependent mannerMacrophage IL-1βHepatitis miceIL-17Liver diseaseControl miceCytokine productionLiver pathologyLiver fibrogenesisMurine modelΑ-SMA
2019
Human induced pluripotent stem cell-derived extracellular vesicles reduce hepatic stellate cell activation and liver fibrosis
Povero D, Pinatel E, Leszczynska A, Goyal N, Nishio T, Kim J, Kneiber D, de Araujo Horcel L, Eguchi A, Ordonez P, Kisseleva T, Feldstein A. Human induced pluripotent stem cell-derived extracellular vesicles reduce hepatic stellate cell activation and liver fibrosis. JCI Insight 2019, 5 PMID: 31184999, PMCID: PMC6675559, DOI: 10.1172/jci.insight.125652.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell MovementCell ProliferationChemical and Drug Induced Liver InjuryChemotaxisDisease Models, AnimalExtracellular VesiclesHepatic Stellate CellsHumansInduced Pluripotent Stem CellsLiverLiver CirrhosisMaleMiceMice, Inbred C57BLMicroRNAsTranscriptomeTransforming Growth Factor betaConceptsHepatic stellate cell activationChronic liver diseaseAnti-fibrotic effectsStellate cell activationLiver fibrosisLiver diseaseExtracellular vesiclesInduced pluripotent stem cellsCell activationDuct ligation-induced liver fibrosisFlow cytometryAnti-fibrotic approachesDevelopment of cirrhosisPro-fibrogenic responseProgression of fibrosisStem cell-derived extracellular vesiclesAnti-fibrotic strategiesCharacterization of iPSCsGreatest unmet needCell-derived extracellular vesiclesMiR-92a-3pWound healing responseStem cellsIPSC-EVsLiver injury