2007
COP1D, an alternatively spliced constitutive photomorphogenic-1 (COP1) product, stabilizes UV stress-induced c-Jun through inhibition of full-length COP1
Savio M, Rotondo G, Maglie S, Rossetti G, Bender J, Pardi R. COP1D, an alternatively spliced constitutive photomorphogenic-1 (COP1) product, stabilizes UV stress-induced c-Jun through inhibition of full-length COP1. Oncogene 2007, 27: 2401-2411. PMID: 17968316, DOI: 10.1038/sj.onc.1210892.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBreast NeoplasmsCell LineDown-RegulationEnzyme StabilityHealthHeLa CellsHumansIsoenzymesJNK Mitogen-Activated Protein KinasesKineticsMolecular Sequence DataProteasome Endopeptidase ComplexRNA, MessengerTranscription, GeneticTumor Suppressor Protein p53Ubiquitin-Protein LigasesConceptsFull-length proteinC-JunCullin-RING ligase complexRING finger ubiquitin ligaseProteasome-dependent degradationDominant-negative functionCoiled-coil regionFunction of COP1Post-translational changesP53 protein levelsLigase complexCRL complexesE3 ligasesE3 ligaseUbiquitin ligaseCOP1Ectopic expressionRNA interferenceUV stressElevated c-JunPolyubiquitinationSplice variants
2006
LFA-1-Dependent HuR Nuclear Export and Cytokine mRNA Stabilization in T Cell Activation
Wang JG, Collinge M, Ramgolam V, Ayalon O, Fan XC, Pardi R, Bender JR. LFA-1-Dependent HuR Nuclear Export and Cytokine mRNA Stabilization in T Cell Activation. The Journal Of Immunology 2006, 176: 2105-2113. PMID: 16455966, DOI: 10.4049/jimmunol.176.4.2105.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAntigens, SurfaceBase SequenceCD28 AntigensCell NucleusCells, CulturedCytokinesCytoplasmELAV ProteinsELAV-Like Protein 1HumansLymphocyte ActivationLymphocyte Function-Associated Antigen-1Molecular Sequence DataRNA StabilityRNA, MessengerRNA, Small InterferingRNA-Binding ProteinsT-LymphocytesConceptsNuclear exportAU-rich element (ARE) sequenceMRNA stabilizationClass II AU-rich elementsT cell activationAU-rich elementsLymphokine gene expressionRapid nuclearRegulated processIntegrin engagementCell activationMRNA reporterRNA interferenceHuR functionIntegrin LFA-1MRNA stabilityGene expressionProtein productionHuman peripheral T cellsCytoplasmic translocationGM-CSF mRNAElement sequencesProtein HuRHuR levelsLFA-1
1999
A tyrosine‐based sorting signal in the β2 integrin cytoplasmic domain mediates its recycling to the plasma membrane and is required for ligand‐supported migration
Fabbri M, Fumagalli L, Bossi G, Bianchi E, Bender J, Pardi R. A tyrosine‐based sorting signal in the β2 integrin cytoplasmic domain mediates its recycling to the plasma membrane and is required for ligand‐supported migration. The EMBO Journal 1999, 18: 4915-4925. PMID: 10487744, PMCID: PMC1171563, DOI: 10.1093/emboj/18.18.4915.Peer-Reviewed Original ResearchConceptsIntegrin cytoplasmic domainCytoplasmic domainBeta-subunit cytoplasmic domainAdhesive functionTyrosine-based endocytic signalsSubunit cytoplasmic domainClathrin-dependent endocytosisSite-directed mutagenesisNon-conservative substitutionsChinese hamster ovary cellsEndocytic signalsRegulated internalizationMembrane rufflesUnrelated functionsRecycling compartmentHamster ovary cellsEctopic expressionPlasma membraneReceptor recyclingDegradative pathwayIntegrin dynamicsCell adhesionCell migrationCell surfaceOvary cellsA MHC-encoded ubiquitin-like protein (FAT10) binds noncovalently to the spindle assembly checkpoint protein MAD2
Liu Y, Pan J, Zhang C, Fan W, Collinge M, Bender J, Weissman S. A MHC-encoded ubiquitin-like protein (FAT10) binds noncovalently to the spindle assembly checkpoint protein MAD2. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 4313-4318. PMID: 10200259, PMCID: PMC16329, DOI: 10.1073/pnas.96.8.4313.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceB-LymphocytesCalcium-Binding ProteinsCarrier ProteinsCell Cycle ProteinsCell LineChromosomes, Artificial, YeastCOS CellsGenes, MHC Class IHL-60 CellsHumansJurkat CellsMad2 ProteinsMolecular Sequence DataPolymerase Chain ReactionRepressor ProteinsSequence AlignmentSequence Homology, Amino AcidT-LymphocytesTransfectionTumor Cells, CulturedUbiquitinsConceptsSpindle assembly checkpoint protein Mad2Two-hybrid screeningCheckpoint protein Mad2Ubiquitin-like proteinHuman MHC class I regionCell cycle checkpointsMHC class I regionSpindle assemblyI geneProtein stabilityCell developmentDendritic cell developmentClass I regionImmunoprecipitation studiesCell growthMad2Gamma-interferon inductionProteinProtein expressionFAT10I regionCellsLymphoblastoid linesAnaphaseTranscriptionHuman single-chain Fv immunoconjugates targeted to a melanoma-associated chondroitin sulfate proteoglycan mediate specific lysis of human melanoma cells by natural killer cells and complement
Wang B, Chen Y, Ayalon O, Bender J, Garen A. Human single-chain Fv immunoconjugates targeted to a melanoma-associated chondroitin sulfate proteoglycan mediate specific lysis of human melanoma cells by natural killer cells and complement. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 1627-1632. PMID: 9990075, PMCID: PMC15540, DOI: 10.1073/pnas.96.4.1627.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell LineCells, CulturedCHO CellsChondroitin Sulfate ProteoglycansChromatography, LiquidComplement System ProteinsCricetinaeCytotoxicity, ImmunologicDrosophila melanogasterEndothelium, VascularHumansImmunoconjugatesImmunoglobulin FragmentsImmunoglobulin GImmunoglobulin Variable RegionKiller Cells, NaturalMass SpectrometryMelanomaMolecular Sequence DataRestriction MappingSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTransfectionTumor Cells, CulturedConceptsHuman melanoma cellsNatural killer cellsNK cellsMelanoma cellsKiller cellsChondroitin sulfate proteoglycanCytolytic responsesMost human melanoma cellsTumor cellsVaccinated melanoma patientsCultured human melanoma cellsCytolytic immune responseFusion phage librarySpecific cytolytic responseMelanoma-associated chondroitin sulfate proteoglycanHuman single-chain FvSulfate proteoglycanMelanoma patientsSpecific lysisImmune responseMelanoma tumorsComplement cascadeTargeted tumor cellsImmunoconjugatesHuman IgG1
1998
Posttranscriptional regulation of urokinase plasminogen activator receptor messenger RNA levels by leukocyte integrin engagement
Wang G, Collinge M, Blasi F, Pardi R, Bender J. Posttranscriptional regulation of urokinase plasminogen activator receptor messenger RNA levels by leukocyte integrin engagement. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 6296-6301. PMID: 9600959, PMCID: PMC27663, DOI: 10.1073/pnas.95.11.6296.Peer-Reviewed Original ResearchConceptsAU-rich elementsLFA-1 activationMRNA degradationReporter constructsUrokinase plasminogen activator receptorT cell activationCrucial cis-acting elementsCis-acting elementsLymphocyte function-associated antigen-1Integrin lymphocyte function-associated antigen-1Jurkat T cellsLFA-1 engagementUPAR cDNAReceptor-mediated signalingPosttranscriptional regulationTransmembrane signalingReporter mRNAIntegrin engagementCell activationInhibitor 5Adhesion receptorsSecond messengerMessenger RNA levelsPlasminogen activator receptorFunction-associated antigen-1
1995
Conserved regions in the cytoplasmic domains of the leukocyte integrin alpha L beta 2 are involved in endoplasmic reticulum retention, dimerization, and cytoskeletal association.
Pardi R, Bossi G, Inverardi L, Rovida E, Bender J. Conserved regions in the cytoplasmic domains of the leukocyte integrin alpha L beta 2 are involved in endoplasmic reticulum retention, dimerization, and cytoskeletal association. The Journal Of Immunology 1995, 155: 1252-63. PMID: 7636193, DOI: 10.4049/jimmunol.155.3.1252.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric RegulationAmino Acid SequenceAnimalsCell CompartmentationCell Line, TransformedChlorocebus aethiopsCytoskeletonEndoplasmic ReticulumLymphocyte Function-Associated Antigen-1Molecular Sequence DataProtein ConformationProtein MultimerizationProtein Structure, TertiaryRecombinant Fusion ProteinsSequence DeletionTetradecanoylphorbol AcetateT-LymphocytesTransfectionConceptsAlpha L beta 2Cytoplasmic domainEndoplasmic reticulum retentionCytoskeletal associationBeta subunitAlpha L subunitAlpha beta complexFunction of integrinsBeta 2GFFKR motifAdherent cellsDeletion mutantsSubstitution mutantsEctopic expressionIntegrin alphaPlasma membraneHeterodimer formationConserved regionsAdhesion receptorsBeta complexCytoplasmic truncationRegulated functionsL subunitsStructural determinantsAlpha LContact-dependent endothelial class II HLA gene activation induced by NK cells is mediated by IFN-gamma-dependent and -independent mechanisms.
Watson C, Petzelbauer P, Zhou J, Pardi R, Bender J. Contact-dependent endothelial class II HLA gene activation induced by NK cells is mediated by IFN-gamma-dependent and -independent mechanisms. The Journal Of Immunology 1995, 154: 3222-33. PMID: 7897208, DOI: 10.4049/jimmunol.154.7.3222.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, Differentiation, B-LymphocyteBase SequenceBlotting, NorthernCell AdhesionCells, CulturedEndothelium, VascularGene Expression RegulationHistocompatibility Antigens Class IIHLA-DR AntigensHumansInterferon-gammaKiller Cells, NaturalMolecular Sequence DataOrgan Culture TechniquesPromoter Regions, GeneticSkinTranscriptional ActivationTransfectionConceptsNK cellsNK lymphocytesEndothelial cellsIFN-gammaMHC class II AgIFN-gamma dependenceT cell recruitmentClass II HLAClass II expressionHLA-DR inductionClass II AgT cell proliferationMembrane expressionTrans-well experimentsReceptor AbEndothelial activationImmune amplificationCell recruitmentMicrovessel endotheliumHuman IFN-gammaPromoter constructsClonal expansionCoculture modelCell proliferationChinese hamster ovary cellsLymphocyte adhesion-dependent calcium signaling in human endothelial cells.
Pfau S, Leitenberg D, Rinder H, Smith BR, Pardi R, Bender JR. Lymphocyte adhesion-dependent calcium signaling in human endothelial cells. Journal Of Cell Biology 1995, 128: 969-978. PMID: 7533170, PMCID: PMC2120392, DOI: 10.1083/jcb.128.5.969.Peer-Reviewed Original ResearchConceptsEC Ca2Endothelial cellsEC alterationsLymphocyte-endothelial interactionsNatural killer cellsImmune effector cellsTumor necrosis factorHuman umbilical vein endothelial cellsCytosolic free calcium changesUmbilical vein endothelial cellsVascular endothelial cellsHuman umbilical vein ECsAllogeneic CD3Lymphocyte subsetsVein endothelial cellsHuman endothelial cellsNK cellsEffector cellsKiller cellsVascular toneInflammatory responseNecrosis factorSecond messenger pathwaysExtracellular influxCytokine activation
1994
Heterogeneous activation thresholds to cytokines in genetically distinct endothelial cells: evidence for diverse transcriptional responses.
Bender JR, Sadeghi MM, Watson C, Pfau S, Pardi R. Heterogeneous activation thresholds to cytokines in genetically distinct endothelial cells: evidence for diverse transcriptional responses. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 3994-3998. PMID: 7513430, PMCID: PMC43709, DOI: 10.1073/pnas.91.9.3994.Peer-Reviewed Original Research