2010
Phosphatidylinositol 4-Phosphate Controls Both Membrane Recruitment and a Regulatory Switch of the Rab GEF Sec2p
Mizuno-Yamasaki E, Medkova M, Coleman J, Novick P. Phosphatidylinositol 4-Phosphate Controls Both Membrane Recruitment and a Regulatory Switch of the Rab GEF Sec2p. Developmental Cell 2010, 18: 828-840. PMID: 20493815, PMCID: PMC2877039, DOI: 10.1016/j.devcel.2010.03.016.Peer-Reviewed Original ResearchConceptsPI4P levelsRab GTPase Sec4pMembrane recruitmentRegulatory switchExchange factorSecretory pathwayVesicle maturationSec2pSec15pSecretory vesiclesPositive feedback loopYpt32pPhosphatidylinositol 4Vesicles formSec4pVesiclesSecretory sitesFeedback loopRecruitment cascadeCascadeRecruitmentPI4PRabPhosphatidylinositolGuanine
2006
The polarity-establishment component Bem1p interacts with the exocyst complex through the Sec15p subunit
France Y, Boyd C, Coleman J, Novick P. The polarity-establishment component Bem1p interacts with the exocyst complex through the Sec15p subunit. Journal Of Cell Science 2006, 119: 876-888. PMID: 16478783, DOI: 10.1242/jcs.02849.Peer-Reviewed Original ResearchConceptsBud growthSrc homology 3 domainTwo-hybrid studiesFirst Src homology 3 domainDirect physical interactionGreen fluorescent proteinExocyst complexGolgi traffickingSec15pSecretory pathwaySpatial regulationBem1pSecretory machineryMaster regulatorFluorescent proteinNew budsMachineryExocystSec4pPhysical interactionSubunitsCase of cellsProteinPathwayCrucial role