2021
Nascent fusion pore opening monitored at single-SNAREpin resolution
Heo P, Coleman J, Fleury JB, Rothman JE, Pincet F. Nascent fusion pore opening monitored at single-SNAREpin resolution. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2024922118. PMID: 33495324, PMCID: PMC7865171, DOI: 10.1073/pnas.2024922118.Peer-Reviewed Original ResearchConceptsFusion poreFusion pore openingTransient fusion poresSingle fusion eventFast neurotransmitter releaseSingle SNAREpinAsymmetric lipid bilayerProtein complexesSNAREpinsTarget membraneFusion eventsCellular traffickingVesicle fusionSmall vesiclesIon channelsLipid bilayersCargo transportKey eventsEnergy landscapePore openingNeurotransmitter releaseDiscrete sizesVesiclesSynaptic transmissionCargo release
2018
High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis
Ramakrishnan S, Gohlke A, Li F, Coleman J, Xu W, Rothman JE, Pincet F. High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis. Langmuir 2018, 34: 5849-5859. PMID: 29694054, DOI: 10.1021/acs.langmuir.8b00116.Peer-Reviewed Original ResearchConceptsMembrane fusionFusion eventsSoluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteinsSNARE-dependent membrane fusionIndividual vesicle fusion eventsFactor attachment protein receptor proteinsN-ethylmaleimide-sensitive factor attachment protein receptor proteinsT-SNARE proteinsSingle-vesicle fusionProtein receptor proteinsVesicle fusion eventsMobility of proteinsVesicle dockingContent releaseVesicle fusionHigh-throughput monitoringPlanar membranesReceptor proteinLipid mixingProteinLipid bilayersVesiclesCorrect reconstitutionMembraneAqueous compartment