Characterization of Within-Host Plasmodium falciparum Diversity Using Next-Generation Sequence Data
Auburn S, Campino S, Miotto O, Djimde A, Zongo I, Manske M, Maslen G, Mangano V, Alcock D, MacInnis B, Rockett K, Clark T, Doumbo O, Ouédraogo J, Kwiatkowski D. Characterization of Within-Host Plasmodium falciparum Diversity Using Next-Generation Sequence Data. PLOS ONE 2012, 7: e32891. PMID: 22393456, PMCID: PMC3290604, DOI: 10.1371/journal.pone.0032891.Peer-Reviewed Original ResearchConceptsHost diversityIllumina GA platformMultiplicity of infectionGenome-wide datasetsPopulation-level diversitySingle-molecule sequencing technologiesNext-generation sequence dataPlasmodium falciparum diversityMsp-1 locusGenetic divergenceSequence dataSequencing technologiesNumber of clonesLevel diversityNovel insightsDiversityP. falciparum fieldLociMSP-1PCRRelative proportionsNew statistical approachSNPsClonesReduced sensitivity