Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres.
Lopez-Ramirez MA, Calvo CF, Ristori E, Thomas JL, Nicoli S. Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. Journal Of Visualized Experiments 2016, 53617. PMID: 26967835, PMCID: PMC4828210, DOI: 10.3791/53617.Peer-Reviewed Original ResearchConceptsAdult zebrafish brainStem/progenitor cellsZebrafish brainMolecular mechanismsRelevant model organismZebrafish adult brainProgenitor cellsSingle-cell dissociationSpecific signaling pathwaysAdult stem/progenitor cellsStem/progenitor cell proliferationNeural stem/progenitor cell proliferationProgenitor cell proliferationNeural stem/progenitor cellsModel organismsGene expressionCell dissociationSignaling pathwaysMultipotent neural stem/progenitor cellsNeurosphere assayAdult neurogenesisCell proliferationZebrafishBrain regenerationAdult brain