2022
CatSper Calcium Channels: 20 Years On
Hwang J, Chung J. CatSper Calcium Channels: 20 Years On. Physiology 2022, 38: 125-140. PMID: 36512352, PMCID: PMC10085559, DOI: 10.1152/physiol.00028.2022.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements3D structure and in situ arrangements of CatSper channel in the sperm flagellum
Zhao Y, Wang H, Wiesehoefer C, Shah NB, Reetz E, Hwang JY, Huang X, Wang TE, Lishko PV, Davies KM, Wennemuth G, Nicastro D, Chung JJ. 3D structure and in situ arrangements of CatSper channel in the sperm flagellum. Nature Communications 2022, 13: 3439. PMID: 35715406, PMCID: PMC9205950, DOI: 10.1038/s41467-022-31050-8.Peer-Reviewed Original ResearchConceptsCatSper complexSperm flagellaCryo-electron tomographyHigher-order organizationMammalian spermIntracellular domainStructural basisExtracellular domainTetrameric channelChannel CatSperSuccessful fertilizationFlagellar movementCatSper channelsChannel poreSitu mapSitu arrangementHuman sperm flagellumPrimary Ca2CatSperDimer formationFlagellaCentral roleSpermAtomic modelSperm motilityCatSper and its CaM‐like Ca2+ sensor EFCAB9 are necessary for the path chirality of sperm
Wiesehöfer C, Wiesehöfer M, Dankert JT, Chung J, von Ostau N, Singer BB, Wennemuth G. CatSper and its CaM‐like Ca2+ sensor EFCAB9 are necessary for the path chirality of sperm. The FASEB Journal 2022, 36: e22288. PMID: 35438819, PMCID: PMC9835897, DOI: 10.1096/fj.202101656rr.Peer-Reviewed Original ResearchConceptsNNC 55Exposure of spermMotility changesPharmacological inhibitionLateral head excursionCatSper activationIntracellular CaPhysiological stimuliCatSper channelsProtein 2Zona pellucida protein 2Zona pellucida glycoproteinsCapacitation stateCatSperHead excursionMouse spermPellucida glycoproteinsSpermSuccessful fertilizationMibefradilHigh frequency
2021
Molecular Evolution of CatSper in Mammals and Function of Sperm Hyperactivation in Gray Short-Tailed Opossum
Hwang JY, Maziarz J, Wagner GP, Chung JJ. Molecular Evolution of CatSper in Mammals and Function of Sperm Hyperactivation in Gray Short-Tailed Opossum. Cells 2021, 10: 1047. PMID: 33946695, PMCID: PMC8147001, DOI: 10.3390/cells10051047.Peer-Reviewed Original ResearchConceptsMolecular evolutionHigh sequence divergenceSperm-specific ion channelIQ-like motifSequence divergenceCatSper activityCatSper subunitsMolecular regulationFertilization environmentSperm hyperactivationCatSper proteinsAmerican marsupialsCatSper functionSequence analysisMarsupialsFemale reproductive tractGray Short-Tailed OpossumsEutheriansSperm flagellaCatSper channelsIon channelsCatSperCurrent understandingOpossumSperm
2020
3D in situ imaging of female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice
Ded L, Hwang JY, Miki K, Shi HF, Chung JJ. 3D in situ imaging of female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice. ELife 2020, 9: e62043. PMID: 33078708, PMCID: PMC7707823, DOI: 10.7554/elife.62043.Peer-Reviewed Original ResearchConceptsFemale reproductive tractFemale tractCalcium channel complexSitu molecular imagingReproductive tractMolecular insightsSuccessful fertilizationCatSper channelsMolecular signaturesSperm cellsSperm selectionChannel complexMouse spermatozoaFertilization siteSpermSperm migrationSpermatozoaMammalsPhosphorylationCatSper1ProteinSitu imagingAcrosomeUtero-tubal junctionSelection
2017
CatSperζ regulates the structural continuity of sperm Ca2+ signaling domains and is required for normal fertility
Chung JJ, Miki K, Kim D, Shim SH, Shi HF, Hwang JY, Cai X, Iseri Y, Zhuang X, Clapham DE. CatSperζ regulates the structural continuity of sperm Ca2+ signaling domains and is required for normal fertility. ELife 2017, 6: e23082. PMID: 28226241, PMCID: PMC5362262, DOI: 10.7554/elife.23082.Peer-Reviewed Original ResearchConceptsMammalian female reproductive tractSevere male subfertilityFemale reproductive tractIon channel complexNovel subunitCatSper complexEvolutionary adaptationMale subfertilityReproductive tractMouse spermSperm Ca2Normal fertilityTargeted disruptionSperm cellsFlagellaChannel complexFemale uterusSubunitsEpididymal spermatozoaSpermComplexesFertilizationSubfertilityUterusMice
2014
Structurally Distinct Ca2+ Signaling Domains of Sperm Flagella Orchestrate Tyrosine Phosphorylation and Motility
Chung JJ, Shim SH, Everley RA, Gygi SP, Zhuang X, Clapham DE. Structurally Distinct Ca2+ Signaling Domains of Sperm Flagella Orchestrate Tyrosine Phosphorylation and Motility. Cell 2014, 157: 808-822. PMID: 24813608, PMCID: PMC4032590, DOI: 10.1016/j.cell.2014.02.056.Peer-Reviewed Original ResearchConceptsTyrosine phosphorylationSperm-specific calcium channelsSpecific protein tyrosine phosphorylationProtein tyrosine phosphorylationSuper-resolution fluorescence microscopyMammalian flagellumUnique molecular phenotypeFlagellar switchHeterogeneous sperm populationsPaternal DNASignaling domainsMolecular phenotypesCatSper channelsMature eggsPhosphorylationFluorescence microscopyDistinct Ca2FlagellaAdaptive strategiesSperm populationCascade organizationSpermUniflagellateCalcium domainsPhosphoproteomics
2008
Ion channels that control fertility in mammalian spermatozoa
Navarro B, Kirichok Y, Chung JJ, Clapham DE. Ion channels that control fertility in mammalian spermatozoa. The International Journal Of Developmental Biology 2008, 52: 607-613. PMID: 18649274, PMCID: PMC4297656, DOI: 10.1387/ijdb.072554bn.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsMammalian spermatozoaGenetic deletion strategiesMammalian spermPhysiological roleIon channelsDeletion strategyIon channel currentsHyperactivated motilityChannel currentsUnambiguous identificationIntracellular alkalinizationWhole-cell voltage clampSpermatozoaGenesTechnical advancesVoltage clampSpermMotilityExpressionFertilizationCa2FertilityIdentification