2022
Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice
Wang H, Dou Q, Jeong KJ, Choi J, Gladyshev VN, Chung JJ. Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice. Journal Of Biological Chemistry 2022, 298: 102077. PMID: 35643315, PMCID: PMC9218152, DOI: 10.1016/j.jbc.2022.102077.Peer-Reviewed Original ResearchConceptsRedox regulationRedox homeostasisMolecular mechanismsSperm maturationMitochondrial sheath formationCell imaging analysisThioredoxin glutathione reductaseSperm developmentMammalian spermatozoaEpididymal transitMitochondrial ultrastructureMitochondrial activityRedox statusMale fertilityCapacitationHomeostasisMaturationRegulationMotilityFamily membersFertilizationSperm morphologySperm motilityMitochondriaBioenergeticsC2cd6-encoded CatSperτ targets sperm calcium channel to Ca2+ signaling domains in the flagellar membrane
Hwang JY, Wang H, Lu Y, Ikawa M, Chung JJ. C2cd6-encoded CatSperτ targets sperm calcium channel to Ca2+ signaling domains in the flagellar membrane. Cell Reports 2022, 38: 110226. PMID: 34998468, PMCID: PMC8857959, DOI: 10.1016/j.celrep.2021.110226.Peer-Reviewed Original ResearchConceptsFlagellar membraneFlagellar membrane targetingC2 domain proteinsCatSper channelsCatSper channel complexMammalian sperm cellsSperm hyperactivated motilityMale fertilityMembrane targetingTrafficking machineryDomain proteinsLoss of functionHyperactivated motilitySpatiotemporal CaChannel assemblyMolecular mechanismsSperm cellsChannel complexFlagellaMotilityC2CD6MembraneCalcium channelsMachineryProtein
2008
Ion channels that control fertility in mammalian spermatozoa
Navarro B, Kirichok Y, Chung JJ, Clapham DE. Ion channels that control fertility in mammalian spermatozoa. The International Journal Of Developmental Biology 2008, 52: 607-613. PMID: 18649274, PMCID: PMC4297656, DOI: 10.1387/ijdb.072554bn.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsMammalian spermatozoaGenetic deletion strategiesMammalian spermPhysiological roleIon channelsDeletion strategyIon channel currentsHyperactivated motilityChannel currentsUnambiguous identificationIntracellular alkalinizationWhole-cell voltage clampSpermatozoaGenesTechnical advancesVoltage clampSpermMotilityExpressionFertilizationCa2FertilityIdentification