2019
Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies
Ali M, Kabir F, Thomson JJ, Ma Y, Qiu C, Delannoy M, Khan SY, Riazuddin SA. Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies. Scientific Reports 2019, 9: 18552. PMID: 31811247, PMCID: PMC6898283, DOI: 10.1038/s41598-019-54258-z.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsComparative transcriptome analysisTranscriptome analysisLentoid bodiesPluripotent stem cellsBody transcriptomeRNA sequencingStem cellsNext-generation RNA sequencingEmbryonic stem cellsFiber-like cellsSimilar expression profilesTranscriptome datasetsTranscriptome profilingCell transcriptomeLens morphogenesisExcellent systemMouse lensExpression profilesTranscriptomeMechanism of cataractogenesisLens-like structuresUltrastructure analysisGenesOcular lens
2018
Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells
Ali M, Khan SY, Vasanth S, Ahmed MR, Chen R, Na CH, Thomson JJ, Qiu C, Gottsch JD, Riazuddin SA. Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells. Investigative Ophthalmology & Visual Science 2018, 59: 2437-2444. PMID: 29847650, PMCID: PMC5957521, DOI: 10.1167/iovs.17-22927.Peer-Reviewed Original ResearchMeSH KeywordsAgedCell DifferentiationCells, CulturedCryopreservationEmbryonic Stem CellsEndothelium, CornealFlow CytometryGene Expression ProfilingGenetic MarkersHumansImmunohistochemistryInduced Pluripotent Stem CellsLeukocytes, MononuclearMaleMass SpectrometryMicroscopy, Phase-ContrastMiddle AgedNeural CrestProteomeReal-Time Polymerase Chain ReactionConceptsNeural crest cellsProteome sequencingProteome profilingCorneal endothelial cellsExpression of pluripotentQuantitative real-time PCRPluripotent stem cellsMolecular architectureCrest cellsEndothelial cellsProteomeReal-time PCRPluripotency markersHuman corneal endotheliumStem cellsPhase contrast microscopyExpression levelsProteinIPSCsSequencingCellsProfilingFirst reportContrast microscopyHigh levels
2014
Tissue‐Engineered Vascular Grafts Created From Human Induced Pluripotent Stem Cells
Sundaram S, One J, Siewert J, Teodosescu S, Zhao L, Dimitrievska S, Qian H, Huang AH, Niklason L. Tissue‐Engineered Vascular Grafts Created From Human Induced Pluripotent Stem Cells. Stem Cells Translational Medicine 2014, 3: 1535-1543. PMID: 25378654, PMCID: PMC4250208, DOI: 10.5966/sctm.2014-0065.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, DifferentiationBlood Vessel ProsthesisCell LineExtracellular MatrixHumansInduced Pluripotent Stem CellsTissue EngineeringConceptsPluripotent stem cellsMesenchymal lineagesSmooth muscle cell differentiationMuscle cell differentiationStem cellsInduced pluripotent stem cellsNeural crest intermediateHuman induced pluripotent stem cellsMesenchymal progenitor cellsStem cell clonesCollagen-rich matrixCell differentiationVascular developmentHiPSC linesProgenitor cellsSmooth muscle cellsCell apoptosisHiPS cellsLineagesMesenchymal markersGeneration of graftMuscle cellsClonesTissue-engineered vascular graftsCells