2005
Human Monocytotropic Ehrlichiosis
Dawson J, Ewing S, Davidson W, Childs J, Little S, Standaert S. Human Monocytotropic Ehrlichiosis. 2005, 239-257. DOI: 10.1128/9781555816490.ch14.Peer-Reviewed Original ResearchHuman monocytotropic ehrlichiosisMonocytotropic ehrlichiosisMild self-limiting symptomsSelf-limiting symptomsNonspecific febrile illnessSpectrum of manifestationsSeasonality of transmissionTick-borne zoonosisFebrile illnessAsymptomatic infectionClinical recognitionHigh riskRickettsial infectionEpidemiological studiesEtiologic agentFatal diseaseLaboratory diagnosisZoonotic diseaseEhrlichia chaffeensisLone star tickE. chaffeensisInfectionDiseaseEhrlichiosisUnited States
2001
Infections with Ehrlichia chaffeensis and Ehrlichia ewingii in Persons Coinfected with Human Immunodeficiency Virus
Paddock C, Folk S, Shore G, Machado L, Huycke M, Slater L, Liddell A, Buller R, Storch G, Monson T, Rimland D, Sumner J, Singleton J, Bloch K, Tang Y, Standaert S, Childs J. Infections with Ehrlichia chaffeensis and Ehrlichia ewingii in Persons Coinfected with Human Immunodeficiency Virus. Clinical Infectious Diseases 2001, 33: 1586-1594. PMID: 11568857, DOI: 10.1086/323981.Peer-Reviewed Original ResearchConceptsHuman immunodeficiency virusImmunodeficiency virusE. ewingiiE. chaffeensisCases of ehrlichiosisDiagnosis of ehrlichiosisT-lymphocyte countsCells/microL.Ehrlichia ewingiiEhrlichia chaffeensisLife-threatening illnessLymphocyte countClinical courseHIV infectionMedical managementPatient cohortSevere manifestationsPatientsLaboratory evaluationEhrlichiosisChaffeensisInfectionEwingiiMultiple factorsVirus
2000
DETECTION OF ANTIBODIES REACTIVE WITH EHRLICHIA CHAFFEENSIS IN THE RACCOON
Comer J, Nicholson W, Paddock C, Sumner J, Childs J. DETECTION OF ANTIBODIES REACTIVE WITH EHRLICHIA CHAFFEENSIS IN THE RACCOON. Journal Of Wildlife Diseases 2000, 36: 705-712. PMID: 11085432, DOI: 10.7589/0090-3558-36.4.705.Peer-Reviewed Original ResearchSurvival of Ehrlichia chaffeensis in refrigerated, ADSOL‐treated RBCs
McKechnie D, Slater K, Childs J, Massung R, Paddock C. Survival of Ehrlichia chaffeensis in refrigerated, ADSOL‐treated RBCs. Transfusion 2000, 40: 1041-1047. PMID: 10988303, DOI: 10.1046/j.1537-2995.2000.40091041.x.Peer-Reviewed Original ResearchConceptsE. chaffeensisNucleated cellsTransfusion-acquired infectionEhrlichia chaffeensisMonocytic cell line THP-1Cell line THP-1Infected cell linesCytocentrifuged slidesTransfusion transmissionPacked RBC unitsPCR positivityE. chaffeensis DNASupernatant fractionCell culturesWeekly examinationsRBC unitsStudy designRBC productsTHP-1DH82 cellsPCR resultsCell linesChaffeensisInfectionSuccessful reisolationPrimary Isolation of Ehrlichia chaffeensis from Patients with Febrile Illnesses: Clinical and Molecular Characteristics
Standaert S, Yu T, Scott M, Childs J, Paddock C, Nicholson W, Singleton J, Blaser M. Primary Isolation of Ehrlichia chaffeensis from Patients with Febrile Illnesses: Clinical and Molecular Characteristics. The Journal Of Infectious Diseases 2000, 181: 1082-1088. PMID: 10720534, DOI: 10.1086/315346.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionE. chaffeensisCerebrospinal fluid specimensCultures of bloodHigh diagnostic yieldEhrlichia chaffeensisPrimary isolationFebrile illnessDiagnostic titerBlood isolatesDiagnostic yieldTick exposureSame patientCerebrospinal fluidPatientsFluid specimensDiagnostic testsFrequent isolationBloodChain reactionChaffeensisInfectionMolecular characteristicsMolecular analysisIsolates
1999
Outcome of Diagnostic Tests Using Samples from Patients with Culture-Proven Human Monocytic Ehrlichiosis: Implications for Surveillance
Childs J, Sumner J, Nicholson W, Massung R, Standaert S, Paddock C. Outcome of Diagnostic Tests Using Samples from Patients with Culture-Proven Human Monocytic Ehrlichiosis: Implications for Surveillance. Journal Of Clinical Microbiology 1999, 37: 2997-3000. PMID: 10449489, PMCID: PMC85432, DOI: 10.1128/jcm.37.9.2997-3000.1999.Peer-Reviewed Original ResearchConceptsHuman monocytic ehrlichiosisDiagnosis of HMEConvalescent-phase serum samplesMonocytic ehrlichiosisDetection of morulaeInitial clinical presentationCulture-proven casesE. chaffeensis antigenWhole blood specimensCompatible illnessClinical presentationInitial presentationSerologic responseCase definitionHME patientsBlood specimensImmunoglobulin MPatientsBlood smearsIndirect immunofluorescenceEhrlichial DNADiagnostic testsSerum samplesProbable diseaseEhrlichia chaffeensisComparison of Methods for Introducing and Producing Artificial Infection of Ixodid Ticks (Acari: Ixodidae) with Ehrlichia chaffeensis
Rechav Y, Zyzak M, Fielden L, Childs J. Comparison of Methods for Introducing and Producing Artificial Infection of Ixodid Ticks (Acari: Ixodidae) with Ehrlichia chaffeensis. Journal Of Medical Entomology 1999, 36: 414-419. PMID: 10467766, DOI: 10.1093/jmedent/36.4.414.Peer-Reviewed Original ResearchDiagnosis of Human Ehrlichiosis by PCR Assay of Acute-Phase Serum
Comer J, Nicholson W, Sumner J, Olson J, Childs J. Diagnosis of Human Ehrlichiosis by PCR Assay of Acute-Phase Serum. Journal Of Clinical Microbiology 1999, 37: 31-34. PMID: 9854059, PMCID: PMC84159, DOI: 10.1128/jcm.37.1.31-34.1999.Peer-Reviewed Original ResearchConceptsDetermination of etiologyAcute-phase seraHuman granulocytic ehrlichiosisSerologic testingHuman ehrlichiosisAcute-phase serum samplesEarly detectionWhole blood specimensPCR assaysBlood specimensPCR testingEtiologic agentGranulocytic ehrlichiosisEtiologyHGE agentSimilar titersSerum samplesExposure historyTitersEhrlichia chaffeensisEhrlichiosisFourfold differenceE. chaffeensisSerumAntibodies
1997
Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis
Paddock C, Sumner J, Shore G, Bartley D, Elie R, McQuade J, Martin C, Goldsmith C, Childs J. Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis. Journal Of Clinical Microbiology 1997, 35: 2496-2502. PMID: 9316896, PMCID: PMC229999, DOI: 10.1128/jcm.35.10.2496-2502.1997.Peer-Reviewed Original ResearchConceptsE. chaffeensisDH82 cellsEhrlichia chaffeensisArkansas isolateGroESL heat shock operonFatal ehrlichiosisMechanisms of diseasePulmonary insufficiencyClinical presentationMarked thrombocytopeniaSevere diseaseEhrlichial infectionCharacteristic manifestationsPatientsDays postinoculationMolecular evaluationChaffeensisPrimary isolationRRNA gene sequencesDiseaseIsolatesSpecific associationNew isolatesGene sequencesProtein gene
1995
Ehrlichiosis in a Golf-Oriented Retirement Community
Standaert S, Dawson J, Schaffner W, Childs J, Biggie K, Singleton J, Gerhardt R, Knight M, Hutcheson R. Ehrlichiosis in a Golf-Oriented Retirement Community. New England Journal Of Medicine 1995, 333: 420-425. PMID: 7616991, DOI: 10.1056/nejm199508173330704.Peer-Reviewed Original ResearchConceptsE. chaffeensis infectionCase-control studySerologic testingCommunity AMedical history reviewLone star tickRisk of infectionCommunity BAnnual incidenceSerologic evidencePolymerase chain reactionTick biteRetirement communityInfectionEhrlichiosisEndemic diseaseChain reactionEhrlichia chaffeensisHigh rateSurvey participantsPercentTicksInsect repellentOutbreakRecreational activities
1994
Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis
Dawson J, Stallknecht D, Howerth E, Warner C, Biggie K, Davidson W, Lockhart J, Nettles V, Olson J, Childs J. Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. Journal Of Clinical Microbiology 1994, 32: 2725-2728. PMID: 7852563, PMCID: PMC264150, DOI: 10.1128/jcm.32.11.2725-2728.1994.Peer-Reviewed Original ResearchConceptsHuman ehrlichiosisE. canisE. chaffeensisEtiologic agentAbsence of seroconversionEhrlichia chaffeensisMacrophage-type cellsE. chaffeensis infectionLymph nodesPeripheral bloodClinical diseaseDay 10InfectionEhrlichia canisEhrlichiosisAntibodiesWhite-tailed deerChaffeensisNegative controlCanisReservoir hostsCompetent reservoirsWeeksTick vectorSeroconversion
1993
Amblyomma americanum: a potential vector of human ehrlichiosis.
Anderson B, Sims K, Olson J, Childs J, Piesman J, Happ C, Maupin G, Johnson B. Amblyomma americanum: a potential vector of human ehrlichiosis. American Journal Of Tropical Medicine And Hygiene 1993, 49: 239-44. PMID: 8357086, DOI: 10.4269/ajtmh.1993.49.239.Peer-Reviewed Original Research