2001
Cl−-dependent secretory mechanisms in isolated rat bile duct epithelial units
Singh S, Mennone A, Gigliozzi A, Fraioli F, Boyer J. Cl−-dependent secretory mechanisms in isolated rat bile duct epithelial units. AJP Gastrointestinal And Liver Physiology 2001, 281: g438-g446. PMID: 11447024, DOI: 10.1152/ajpgi.2001.281.2.g438.Peer-Reviewed Original ResearchMeSH KeywordsAmmonium ChlorideAnimalsBariumBicarbonatesBile Ducts, IntrahepaticBumetanideCarbon DioxideCarrier ProteinsCell PolarityCells, CulturedChloridesColforsinEpithelial CellsHydrogen-Ion ConcentrationIon TransportMaleModels, BiologicalRatsRats, Sprague-DawleySodiumSodium-Potassium-Chloride SymportersConceptsCO2/HCOFluid secretionCotransport activityIntrahepatic bile duct epitheliumBile duct epitheliumDependent fluid secretionHigh intracellular Cl(-) concentrationIntracellular Cl- concentrationBile duct unitsApical Cl(-) secretionNH entryInitial acidification rateIntracellular cAMP concentrationNH4Cl exposureDuct epitheliumInhibitor bumetanideChannel inhibitorsCl(-) secretionCanalicular bileDuct unitsSecretionDependent secretionSecretory mechanismBumetanideForskolinIsolation of functional polarized bile duct units from mouse liver
Cho W, Mennone A, Boyer J. Isolation of functional polarized bile duct units from mouse liver. AJP Gastrointestinal And Liver Physiology 2001, 280: g241-g246. PMID: 11208546, DOI: 10.1152/ajpgi.2001.280.2.g241.Peer-Reviewed Original ResearchConceptsVasoactive intestinal peptideBile duct epitheliumMurine animal modelBile duct unitsPortal perfusionIntestinal peptideSecretory responseAnimal modelsBlunt dissectionDuct epitheliumSecretory stimuliFluid secretionDuct unitsCholangiocyte secretionFurther enzymatic digestionMouse liverSecretionMurine animalsIBDUMice
1999
Characterization of ion transport mechanisms involved in bombesin-stimulated biliary secretion in rat cholangiocytes
Cho W, Boyer J. Characterization of ion transport mechanisms involved in bombesin-stimulated biliary secretion in rat cholangiocytes. Journal Of Hepatology 1999, 30: 1045-1051. PMID: 10406182, DOI: 10.1016/s0168-8278(99)80258-x.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAcetazolamideAnimalsBarium CompoundsBileBile DuctsBombesinCarbonic Anhydrase InhibitorsChloride ChannelsChloridesIn Vitro TechniquesIon TransportMaleMicroscopy, VideoNitrobenzoatesPotassium ChannelsRatsRats, Sprague-DawleyTetraethylammonium
1998
Intracellular pH regulation in bombesin-stimulated secretion in isolated bile duct units from rat liver
Cho W, Mennone A, Boyer J. Intracellular pH regulation in bombesin-stimulated secretion in isolated bile duct units from rat liver. American Journal Of Physiology 1998, 275: g1028-g1036. PMID: 9815033, DOI: 10.1152/ajpgi.1998.275.5.g1028.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiportersBicarbonatesBile Ducts, IntrahepaticBombesinCarrier ProteinsChloride-Bicarbonate AntiportersChloridesFluoresceinsFluorescent DyesHEPESHydrogen-Ion ConcentrationIn Vitro TechniquesMaleModels, BiologicalRatsRats, Sprague-DawleySodiumSodium-Bicarbonate SymportersSodium-Hydrogen ExchangersConceptsHCO-3 secretionBile duct unitsDuct unitsAcid-extruding mechanismsEffect of bombesinRat liverCl-/HCOBombesin-stimulated secretionRegulation of intracellularBiliary secretionNormal rat liverBombesinCholangiocytesSecretionUnderlying mechanismLiverBasalPH regulationCounterregulatoryNeuropeptidesExchange activityActivity
1996
Swelling-activated anion conductance in skate hepatocytes: regulation by cell Cl- and ATP
Jackson P, Churchwell K, Ballatori N, Boyer J, Strange K. Swelling-activated anion conductance in skate hepatocytes: regulation by cell Cl- and ATP. American Journal Of Physiology 1996, 270: c57-c66. PMID: 8772430, DOI: 10.1152/ajpcell.1996.270.1.c57.Peer-Reviewed Original Research
1995
Isolation of small polarized bile duct units.
Mennone A, Alvaro D, Cho W, Boyer J. Isolation of small polarized bile duct units. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 6527-6531. PMID: 7541542, PMCID: PMC41551, DOI: 10.1073/pnas.92.14.6527.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesBile DuctsBucladesineChloridesCholestasisColforsinFluoresceinsGluconatesHydrogen-Ion ConcentrationKineticsLiverMaleMicroscopy, ElectronMicroscopy, VideoRatsRats, Sprague-DawleySecretinTime FactorsConceptsBile duct unitsInterlobular bile ductsBile ductDuct unitsSmall interlobular bile ductsBile duct syndromeBile duct segmentsCholestatic liver disordersCl-/HCO3- exchange activityRat liverLiver disordersPrimary siteForskolin administrationMicroM forskolinDextran 40Duct segmentsDibutyryl cAMPUseful preparationLiverSecretionForskolinImaging techniquesDuctLuminal spaceSyndrome
1994
Regulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes.
Benedetti A, Strazzabosco M, Ng O, Boyer J. Regulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 792-796. PMID: 8290601, PMCID: PMC43035, DOI: 10.1073/pnas.91.2.792.Peer-Reviewed Original ResearchConceptsApical targetingExchange activityRegulation of activityMembrane localizationCl-/HCO3Dibutyryl cAMPProtein kinase C agonistsRat hepatocytesProtein kinase C agonist phorbolEcto-ATPaseConfocal microscopyCanalicular domainC agonistsProteinVesiclesTargetingCanalicular localizationLocalizationCAMPHepatocyte coupletsHepatocytesActivityRegulationPhorbolColchicine
1993
Effect of ursodeoxycholic acid on intracellular pH regulation in isolated rat bile duct epithelial cells
Alvaro D, Mennone A, Boyer J. Effect of ursodeoxycholic acid on intracellular pH regulation in isolated rat bile duct epithelial cells. American Journal Of Physiology 1993, 265: g783-g791. PMID: 8238362, DOI: 10.1152/ajpgi.1993.265.4.g783.Peer-Reviewed Original ResearchConceptsBile duct epithelial cellsUrsodeoxycholic acidDuct epithelial cellsBDE cellsHCO3- excretionEffect of UDCAEpithelial cellsHCO3- exchangeCholic acidKrebs-Ringer bicarbonateUDCA infusionUDCA withdrawalRat bile duct epithelial cellsKRB mediumHCO3- symportNormal rat liverIntracellular pH regulationHCO3- secretionSodium tauroursodeoxycholateSecondary activationBasal levelsPresence of HCO3TauroursodeoxycholateRat liverHypercholeresisEffect of secretion on intracellular pH regulation in isolated rat bile duct epithelial cells.
Alvaro D, Cho W, Mennone A, Boyer J. Effect of secretion on intracellular pH regulation in isolated rat bile duct epithelial cells. Journal Of Clinical Investigation 1993, 92: 1314-1325. PMID: 8397224, PMCID: PMC288273, DOI: 10.1172/jci116705.Peer-Reviewed Original ResearchConceptsBile duct epithelial cellsDuct epithelial cellsCl-/HCO3Cell depolarizationEpithelial cellsEffect of secretinCl- channel blockerEffects of secretionsCl removalCl- channel activationRat bile duct epithelial cellsKRB mediumMaximal rateRegulation of intracellularBicarbonate secretionNormal rat liverIntracellular pH regulationMM DIDSHCO3- excretionAcid loadBDE cellsSecretinMicrofluorimetric methodRat liverHCO3- influx
1991
Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH
Benedetti A, Strazzabosco M, Corasanti J, Haddad P, Graf J, Boyer J. Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH. American Journal Of Physiology 1991, 261: g512-g522. PMID: 1887897, DOI: 10.1152/ajpgi.1991.261.3.g512.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAmmoniaAnimalsBariumBicarbonatesCarrier ProteinsCells, CulturedChloride-Bicarbonate AntiportersChloridesHydrogen-Ion ConcentrationIntracellular FluidKineticsLiverMaleRatsRats, Inbred StrainsConceptsAlkaline loadPH-sensitive dye BCECFMM H2DIDSPHi recoveryRegulation of intracellularRat hepatocyte monolayersAcid extrudersMM DIDSRat hepatocytesElectrophysiological techniquesDye BCECFHCO3- exchangerIsolated rat hepatocytesConstant pHoIntracellularMembrane potentialHepatocyte monolayersHepatocytesAcid loadingRole of chloride ions in liver cell volume regulation
Haddad P, Beck J, Boyer J, Graf J. Role of chloride ions in liver cell volume regulation. American Journal Of Physiology 1991, 261: g340-g348. PMID: 1872402, DOI: 10.1152/ajpgi.1991.261.2.g340.Peer-Reviewed Original ResearchConceptsRegulatory volume decreaseM omegaLiver cellsSingle isolated rat hepatocytesBiphasic responseHypotonic stressInitial hyperpolarizationPl/minIsolated rat hepatocytesGradual depolarizationCell volume regulationExternal ClRole of C1Hypotonic swellingVolume regulationMembrane potentialFacilitated releaseRat hepatocytesCellsMinVolume decreaseCell volumeInitial volumeInitial rateLiver
1990
Effects of osmotic stresses on isolated rat hepatocytes. I. Ionic mechanisms of cell volume regulation
Corasanti J, Gleeson D, Boyer J. Effects of osmotic stresses on isolated rat hepatocytes. I. Ionic mechanisms of cell volume regulation. American Journal Of Physiology 1990, 258: g290-g298. PMID: 2305895, DOI: 10.1152/ajpgi.1990.258.2.g290.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAmilorideAnimalsBariumBicarbonatesBumetanideCells, CulturedChloridesHypertonic SolutionsHypotonic SolutionsKineticsLiverMaleOsmolar ConcentrationPotassium ChlorideQuinineRatsRats, Inbred StrainsSodium Chloride
1987
Cell membrane and transepithelial voltages and resistances in isolated rat hepatocyte couplets
Graf J, Henderson R, Krumpholz B, Boyer J. Cell membrane and transepithelial voltages and resistances in isolated rat hepatocyte couplets. The Journal Of Membrane Biology 1987, 95: 241-254. PMID: 3585979, DOI: 10.1007/bf01869486.Peer-Reviewed Original Research
1985
Evidence for carrier-mediated chloride/bicarbonate exchange in canalicular rat liver plasma membrane vesicles.
Meier P, Knickelbein R, Moseley R, Dobbins J, Boyer J. Evidence for carrier-mediated chloride/bicarbonate exchange in canalicular rat liver plasma membrane vesicles. Journal Of Clinical Investigation 1985, 75: 1256-1263. PMID: 2985654, PMCID: PMC425453, DOI: 10.1172/jci111824.Peer-Reviewed Original ResearchMechanisms of Electrolyte Transport in the Liver Their Functional Significance
Moseley R, Boyer J. Mechanisms of Electrolyte Transport in the Liver Their Functional Significance. Seminars In Liver Disease 1985, 5: 122-135. PMID: 3161181, DOI: 10.1055/s-2008-1063917.Peer-Reviewed Original Research
1984
Pardaxin increases solute permeability of gills and rectal gland in the dogfish shark (Squalus acanthias)
Primor N, Zadunaisky J, Murdaugh H, Boyer J, Forrest J. Pardaxin increases solute permeability of gills and rectal gland in the dogfish shark (Squalus acanthias). Comparative Biochemistry And Physiology Part C Toxicology & Pharmacology 1984, 78: 483-490. PMID: 6149101, DOI: 10.1016/0742-8413(84)90119-1.Peer-Reviewed Original Research
1982
Structure of tight junctions during Cl secretion in the perfused rectal gland of the dogfish shark
Forrest J, Boyer J, Ardito T, Murdaugh H, Wade J. Structure of tight junctions during Cl secretion in the perfused rectal gland of the dogfish shark. American Journal Of Physiology 1982, 242: c388-c392. PMID: 7081428, DOI: 10.1152/ajpcell.1982.242.5.c388.Peer-Reviewed Original Research