A Novel Di-Leucine Motif at the N-Terminus of Human Organic Solute Transporter Beta Is Essential for Protein Association and Membrane Localization
Xu S, Soroka CJ, Sun AQ, Backos DS, Mennone A, Suchy FJ, Boyer JL. A Novel Di-Leucine Motif at the N-Terminus of Human Organic Solute Transporter Beta Is Essential for Protein Association and Membrane Localization. PLOS ONE 2016, 11: e0158269. PMID: 27351185, PMCID: PMC4924846, DOI: 10.1371/journal.pone.0158269.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsCell MembraneHEK293 CellsHumansHydrophobic and Hydrophilic InteractionsMembrane Transport ProteinsMutationProtein BindingProtein DomainsProtein TransportConceptsMembrane localizationAlpha subunitBeta subunitDileucine motifPlasma membrane localizationDi-leucine motifExtracellular N-terminal regionCo-immunoprecipitation studiesBasolateral membrane localizationN-terminal regionSite-directed mutagenesisAA mutantProtein associationBeta mutantsN-terminusMultiple speciesPhysical associationMembrane biotinylationHEK293 cellsSubunitsAA mutationsMembrane expressionAlpha/betaMutantsMotif