2011
Ostα depletion protects liver from oral bile acid load
Soroka CJ, Velazquez H, Mennone A, Ballatori N, Boyer JL. Ostα depletion protects liver from oral bile acid load. AJP Gastrointestinal And Liver Physiology 2011, 301: g574-g579. PMID: 21719738, PMCID: PMC3174539, DOI: 10.1152/ajpgi.00141.2011.Peer-Reviewed Original ResearchConceptsExcess bile acidsCholic acid feedingWild-type miceBile acid overloadBile acidsLiver injuryAcid overloadLower serum ALT levelsIntestinal bile acid absorptionAcid feedingBile acid loadSerum ALT levelsBile acid absorptionBile acid lossBile duct ligationEffective therapeutic targetBile acid homeostasisWild-type controlsALT levelsUrinary eliminationIntestinal lossObstructive cholestasisIntestinal functionDuct ligationUrinary clearance
2001
Adaptive regulation of bile salt transporters in kidney and liver in obstructive cholestasis in the rat
Lee J, Azzaroli F, Wang L, Soroka C, Gigliozzi A, Setchell K, Kramer W, Boyer J. Adaptive regulation of bile salt transporters in kidney and liver in obstructive cholestasis in the rat. Gastroenterology 2001, 121: 1473-1484. PMID: 11729126, DOI: 10.1053/gast.2001.29608.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAnimalsBile Acids and SaltsCarrier ProteinsCholestasisCommon Bile DuctFluorescent Antibody TechniqueKidneyLigationLiverMaleMicrovilliMitochondrial ProteinsOrganic Anion Transporters, Sodium-DependentRatsRats, Sprague-DawleyRibosomal ProteinsRNA, MessengerSaccharomyces cerevisiae ProteinsSymportersConceptsBile salt excretionCommon bile duct ligationBile salt transportersBile duct ligationSalt excretionObstructive cholestasisSalt transportersDuct ligationCommon bile duct obstructionKidney 14 daysBile duct obstructionBile salt transport proteinsSerum bile saltsBrush border membrane vesiclesProtein 2 expressionBile salt transportSodium-dependent uptakeExtrahepatic pathwaysLiver injuryDuct obstructionTissue immunofluorescenceBorder membrane vesiclesTransporter messenger RNAExtrahepatic tissuesTotal liver
2000
Expression of the bile salt export pump is maintained after chronic cholestasis in the rat
Lee J, Trauner M, Soroka C, Stieger B, Meier P, Boyer J. Expression of the bile salt export pump is maintained after chronic cholestasis in the rat. Gastroenterology 2000, 118: 163-172. PMID: 10611165, DOI: 10.1016/s0016-5085(00)70425-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsATP Binding Cassette Transporter, Subfamily B, Member 1ATP Binding Cassette Transporter, Subfamily B, Member 11ATP-Binding Cassette TransportersBile Acids and SaltsBile DuctsCarrier ProteinsCholestasisChronic DiseaseDrug Resistance, MultipleEthinyl EstradiolLigationLipopolysaccharidesMembrane ProteinsMultidrug Resistance-Associated ProteinsOrganic Anion Transporters, Sodium-DependentRatsRats, Sprague-DawleyRNA, MessengerSymporters
1998
Maternal cholestasis does not affect the ontogenic pattern of expression of the Na+/Taurocholate cotransporting polypeptide (ntcp) in the fetal and neonatal rat liver
Arrese M, Trauner M, Ananthanarayanan M, Boyer J, Suchy F. Maternal cholestasis does not affect the ontogenic pattern of expression of the Na+/Taurocholate cotransporting polypeptide (ntcp) in the fetal and neonatal rat liver. Hepatology 1998, 28: 789-795. PMID: 9731574, DOI: 10.1002/hep.510280328.Peer-Reviewed Original ResearchConceptsBile duct ligationWeeks of ageBile acid transporterFetal lifeCommon bile duct ligationOntogenic patternMaternal obstructive cholestasisBile acid excretionMRNA levelsEffect of cholestasisSham-operated animalsSteady-state mRNA levelsFunction of NTCPAcid transportersNeonatal rat liverPostnatal time pointsDays of ageMaternal cholestasisBasolateral bile acid transportersPregnant ratsCholestatic ratsLiver weightObstructive cholestasisDuct ligationMC groupEndotoxin downregulates rat hepatic ntcp gene expression via decreased activity of critical transcription factors.
Trauner M, Arrese M, Lee H, Boyer J, Karpen S. Endotoxin downregulates rat hepatic ntcp gene expression via decreased activity of critical transcription factors. Journal Of Clinical Investigation 1998, 101: 2092-2100. PMID: 9593765, PMCID: PMC508797, DOI: 10.1172/jci1680.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBile Acids and SaltsCarrier ProteinsCells, CulturedCholestasisDNA-Binding ProteinsDown-RegulationEndotoxinsGene Expression RegulationHepatocyte Nuclear Factor 1Hepatocyte Nuclear Factor 1-alphaHepatocyte Nuclear Factor 1-betaLiverMaleMembrane Transport ProteinsNuclear ProteinsOrganic Anion Transporters, Sodium-DependentPromoter Regions, GeneticRatsRats, Sprague-DawleyRNA, MessengerSepsisSymportersTranscription FactorsConceptsNuclear binding activityNtcp mRNA levelsMRNA levelsHepatobiliary transportersMRNA expressionCritical transcription factorTime pointsSepsis-associated cholestasisNuclear factor-kappaBSodium-dependent uptakeEffector cytokinesSequential time pointsEndotoxin administrationPretreatment levelsBinding activityMaximal decreaseBile acidsFactor-kappaBHepatocyte nuclear factor 1Normal levelsHepatic basolateral membraneNuclear factorMarked reductionCoordinated downregulationEndotoxin
1997
Expression of the rat liver Na+/taurocholate cotransporter is regulated in vivo by retention of biliary constituents but not their depletion
Gartung C, Schuele S, Schlosser S, Boyer J. Expression of the rat liver Na+/taurocholate cotransporter is regulated in vivo by retention of biliary constituents but not their depletion. Hepatology 1997, 25: 284-290. PMID: 9021935, DOI: 10.1002/hep.510250205.Peer-Reviewed Original Research
1996
Down-regulation of expression and function of the rat liver Na+/bile acid cotransporter in extrahepatic cholestasis
Gartung C, Ananthanarayanan M, Rahman M, Schuele S, Nundy S, Soroka C, Stolz A, Suchy F, Boyer J. Down-regulation of expression and function of the rat liver Na+/bile acid cotransporter in extrahepatic cholestasis. Gastroenterology 1996, 110: 199-209. PMID: 8536857, DOI: 10.1053/gast.1996.v110.pm8536857.Peer-Reviewed Original ResearchMeSH Keywords3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)3-Hydroxysteroid DehydrogenasesAnimalsCarrier ProteinsCholestasis, ExtrahepaticCommon Bile DuctDown-RegulationHomeostasisLigationLiverMaleOrganic Anion Transporters, Sodium-DependentProteinsRatsRats, Sprague-DawleyRNA, MessengerSymportersTaurocholic AcidTissue DistributionTranscription, GeneticConceptsEcto-adenosine triphosphataseRegulation of expressionMolecular regulationPosttranscriptional regulationGene transcriptionSteady-state messenger RNA levelsTranscriptional activityAcid transportersProtective feedback mechanismComplementary DNAAcid cotransporterMessenger RNA levelsSodium-dependent taurocholateTranscriptionRNA levelsRegulationBile acid transporterMRNA levelsHepatic bile acid transportersCanalicular localizationTaurocholate uptakeAlpha-hydroxysteroid dehydrogenaseExpressionNtcp proteinTriphosphatase
1994
Characterization of cloned rat liver Na(+)-bile acid cotransporter using peptide and fusion protein antibodies
Ananthanarayanan M, Ng O, Boyer J, Suchy F. Characterization of cloned rat liver Na(+)-bile acid cotransporter using peptide and fusion protein antibodies. American Journal Of Physiology 1994, 267: g637-g643. PMID: 7943329, DOI: 10.1152/ajpgi.1994.267.4.g637.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesBase SequenceBlotting, WesternCarrier ProteinsCloning, MolecularFluorescent Antibody TechniqueHumansImmune SeraImmunoblottingLiverMaleMolecular ProbesMolecular Sequence DataOrganic Anion Transporters, Sodium-DependentPeptide FragmentsRatsRats, Sprague-DawleyRecombinant Fusion ProteinsSymportersExpression and characterization of a functional rat liver Na+ bile acid cotransport system in COS-7 cells
Boyer J, Ng O, Ananthanarayanan M, Hofmann A, Schteingart C, Hagenbuch B, Stieger B, Meier P. Expression and characterization of a functional rat liver Na+ bile acid cotransport system in COS-7 cells. American Journal Of Physiology 1994, 266: g382-g387. PMID: 8166278, DOI: 10.1152/ajpgi.1994.266.3.g382.Peer-Reviewed Original ResearchConceptsCOS-7 cellsCotransport systemBile acid transporterUrsodeoxycholic acidTaurochenodeoxycholic acidAcid cotransport systemTauroursodeoxycholic acidBile acidsRat hepatocytesAbsence of sodiumChenodeoxycholic acidCOS cellsEmpty plasmidPMAMneo vectorCholic acidBile acid cotransporterMicroM bilirubinRat liverEvidence of expressionCell linesAcid transportersPosttranslational factorsProgressive uptakeCotransporterAcid cotransporter
1993
Phylogenic and ontogenic expression of hepatocellular bile acid transport.
Boyer J, Hagenbuch B, Ananthanarayanan M, Suchy F, Stieger B, Meier P. Phylogenic and ontogenic expression of hepatocellular bile acid transport. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 435-438. PMID: 8421672, PMCID: PMC45677, DOI: 10.1073/pnas.90.2.435.Peer-Reviewed Original ResearchConceptsAcid transportMammalian speciesFull-length cDNA probeDependent bile acid transportMammalian fetal developmentAcid cotransport systemVertebrate evolutionBile acid transportOntogenic expressionLower vertebratesNonmammalian speciesPrior functional studiesHepatoma cell lineNorthern analysisAcid transportersDedifferentiated hepatocytesCDNA probeTransport systemAcid cotransporterFunctional studiesXenopus oocytesSmall skateSpeciesMRNACell lines
1991
Mechanism of mercurial inhibition of sodium-coupled alanine uptake in liver plasma membrane vesicles from Raja erinacea
Sellinger M, Ballatori N, Boyer J. Mechanism of mercurial inhibition of sodium-coupled alanine uptake in liver plasma membrane vesicles from Raja erinacea. Toxicology And Applied Pharmacology 1991, 107: 369-376. PMID: 1994517, DOI: 10.1016/0041-008x(91)90216-2.Peer-Reviewed Original ResearchConceptsDependent alanine uptakePlasma membrane vesiclesMembrane vesiclesAlanine uptakePrimitive vertebratesPlasma membraneTransport proteinsConcentration-dependent mechanismLiver plasma membrane vesiclesMicroM HgCl2Mammalian hepatocytesAlanine carrierSulfhydryl groupsLittle skateMembrane integrityRaja erinaceaDirect interactionVesiclesVesicle integrityMembrane permeabilityMercurial inhibitionUptake rateDose-dependent mannerHgCl2 concentrationsLiver plasma membranes