2013
Bile Formation and Secretion
Boyer JL. Bile Formation and Secretion. 2013, 3: 1035-1078. PMID: 23897680, PMCID: PMC4091928, DOI: 10.1002/cphy.c120027.Peer-Reviewed Original Research
2006
The organic anion transport polypeptide 1d1 (Oatp1d1) mediates hepatocellular uptake of phalloidin and microcystin into skate liver
Meier-Abt F, Hammann-Hänni A, Stieger B, Ballatori N, Boyer J. The organic anion transport polypeptide 1d1 (Oatp1d1) mediates hepatocellular uptake of phalloidin and microcystin into skate liver. Toxicology And Applied Pharmacology 2006, 218: 274-279. PMID: 17198718, DOI: 10.1016/j.taap.2006.11.015.Peer-Reviewed Original ResearchConceptsSkate liverToxic liver injuryFunctional transport activityLiver-specific OATPsOrganic anion transporting polypeptidesLiver injuryOrganic anion transportersWater-injected oocytesXenobiotic toxinsHepatocellular uptakeBile saltsSkate hepatocytesOATP substratesHuman brainLiverPresent studyOatps/OATPsAnion transportersOATPDemethylphalloinHepatocytesTransport activityToxinCellular uptakeUptake
2001
Bile salt export pump is highly conserved during vertebrate evolution and its expression is inhibited by PFIC type II mutations
Cai S, Wang L, Ballatori N, Boyer J. Bile salt export pump is highly conserved during vertebrate evolution and its expression is inhibited by PFIC type II mutations. AJP Gastrointestinal And Liver Physiology 2001, 281: g316-g322. PMID: 11447010, DOI: 10.1152/ajpgi.2001.281.2.g316.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsATP Binding Cassette Transporter, Subfamily B, Member 11ATP-Binding Cassette TransportersBiological TransportCholestasis, IntrahepaticCloning, MolecularConserved SequenceEvolution, MolecularHumansLiverMolecular Sequence DataMutationPhylogenyRNA, MessengerSequence Homology, Amino AcidSkates, FishSpodopteraTaurocholic AcidTransfectionConceptsSf9 cellsATP-dependent transport proteinsFull-length cloneATP-dependent export pumpLiver cDNA libraryNorthern blot analysisStructure-function relationshipsVertebrate evolutionEvolutionary originHuman BSEPBile salt export pump BSEPMutant proteinsSkate Raja erinaceaHigher vertebratesCDNA libraryTransport proteinsSixfold stimulationVertebratesType II mutationAmino acidsRaja erinaceaDefective expressionBlot analysisExport pumpMutations
2000
ATP-dependent GSH and glutathioneS-conjugate transport in skate liver: role of an Mrp functional homologue
Rebbeor J, Connolly G, Henson J, Boyer J, Ballatori N. ATP-dependent GSH and glutathioneS-conjugate transport in skate liver: role of an Mrp functional homologue. AJP Gastrointestinal And Liver Physiology 2000, 279: g417-g425. PMID: 10915652, DOI: 10.1152/ajpgi.2000.279.2.g417.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsAnion Transport ProteinsAnionsATP-Binding Cassette TransportersBileBiological TransportCarrier ProteinsCell MembraneEnzyme InhibitorsGlutathioneLiverMaleOxidation-ReductionProtein BindingRatsRats, Sprague-DawleySkates, FishSubstrate SpecificityTaurocholic AcidTritiumConceptsATP-dependent transportLiver membrane vesiclesATP-dependent GSH transportMembrane vesiclesTransport activityATP-independent mechanismGlutathione-conjugate transportFunctional homologueMammalian cellsDNP-SG uptakePlasma membraneHomologous proteinsSkate liverGSH transportLow-affinity pathwayLittle skateMRP familyMultidrug resistance-associated protein 1GSH effluxFunctional assaysProtein 1First direct evidenceProtein bandsDirect evidenceHomologuesBile salt excretion in skate liver is mediated by a functional analog of Bsep/Spgp, the bile salt export pump
Ballatori N, Rebbeor J, Connolly G, Seward D, Lenth B, Henson J, Sundaram P, Boyer J. Bile salt excretion in skate liver is mediated by a functional analog of Bsep/Spgp, the bile salt export pump. AJP Gastrointestinal And Liver Physiology 2000, 278: g57-g63. PMID: 10644562, DOI: 10.1152/ajpgi.2000.278.1.g57.Peer-Reviewed Original ResearchConceptsTransport activityPlasma membrane vesiclesApparent molecular massVertebrate evolutionATP-dependent componentMultidrug resistance protein 1Similar transport activityFunctional characterizationPlasma membraneApical localizationCanalicular membraneResistance protein 1Liver plasma membrane vesiclesATP-dependent uptakeSkate liverMembrane vesiclesWestern blot analysisDominant protein bandsMammalian liverMolecular massLittle skateFunctional analogueProtein 1Raja erinaceaCanalicular multidrug resistance
1999
Carrier-mediated uptake of lucifer yellow in skate and rat hepatocytes: a fluid-phase marker revisited
Ballatori N, Hager D, Nundy S, Miller D, Boyer J. Carrier-mediated uptake of lucifer yellow in skate and rat hepatocytes: a fluid-phase marker revisited. American Journal Of Physiology 1999, 277: g896-g904. PMID: 10516157, DOI: 10.1152/ajpgi.1999.277.4.g896.Peer-Reviewed Original ResearchCanalicular Export Pumps Traffic with Polymeric Immunoglobulin A Receptor on the Same Microtubule-associated Vesicle in Rat Liver*
Soroka C, Pate M, Boyer J. Canalicular Export Pumps Traffic with Polymeric Immunoglobulin A Receptor on the Same Microtubule-associated Vesicle in Rat Liver*. Journal Of Biological Chemistry 1999, 274: 26416-26424. PMID: 10473600, DOI: 10.1074/jbc.274.37.26416.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBile CanaliculiBiological TransportBiopolymersCells, CulturedLiverMicroscopy, ElectronMicrotubulesRatsReceptors, FcConceptsApical membrane proteinsMembrane proteinsMicrotubule-associated vesiclesSite of functionMembrane transport proteinsMembrane-bound vesiclesNon-hydrolyzable nucleotidesReceptor trafficMicrotubule motorsTransport proteinsIntermediate chainEssential pathwayPolymeric immunoglobulinsSame vesiclesTranscytotic vesiclesMultidrug resistance protein 2Protein 2Immunoelectron microscopyVesiclesMicrotubulesProteinVesicular transcytosisPathwayRat hepatocytesCanalicular exportMolecular regulation of hepatocellular transport systems in cholestasis
Trauner M, Meier P, Boyer J. Molecular regulation of hepatocellular transport systems in cholestasis. Journal Of Hepatology 1999, 31: 165-178. PMID: 10424299, DOI: 10.1016/s0168-8278(99)80179-2.Peer-Reviewed Original ResearchHepatic sequestration and modulation of the canalicular transport of the organic cation, daunorubicin, in the rat
Hayes J, Soroka C, Rios‐Velez L, Boyer J. Hepatic sequestration and modulation of the canalicular transport of the organic cation, daunorubicin, in the rat. Hepatology 1999, 29: 483-493. PMID: 9918926, DOI: 10.1002/hep.510290216.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsAntineoplastic AgentsATP Binding Cassette Transporter, Subfamily B, Member 1BileBile CanaliculiBiological TransportBucladesineCationsChloroquineDaunorubicinKineticsLiverMacrolidesMaleMitochondrial ProteinsNocodazoleRatsRats, WistarRibosomal ProteinsSaccharomyces cerevisiae ProteinsTaurocholic AcidVerapamilConceptsBiliary excretionCoadministration of verapamilInhibitors of MDR1Rat hepatocyte coupletsBiliary recoveryDose administeredHepatic sequestrationTR- mutant ratsTherapeutic agentsExcretionDaunorubicinPericanalicular regionHepatocyte coupletsCanalicular transportRat liverBafilomycin ATaurocholateRatsIPRLIntracellular acidic compartmentsCellular localizationMicrotubule destabilizerConfocal microscopyIRHCPattern of distributionModulation of protein kinase C by taurolithocholic acid in isolated rat hepatocytes
Beuers U, Probst I, Soroka C, Boyer J, Kullak‐Ublick G, Paumgartner G. Modulation of protein kinase C by taurolithocholic acid in isolated rat hepatocytes. Hepatology 1999, 29: 477-482. PMID: 9918925, DOI: 10.1002/hep.510290227.Peer-Reviewed Original Research
1998
Advances in hepatic transport: Molecular mechanisms, genetic disorders, and treatment. A summary of the 1998 AASLD single topic conference
Wolkoff A, Suchy F, Moseley R, Meier P, Gollan J, Freimer N, Fitz J, Boyer J, Berk P, Scharschmidt B. Advances in hepatic transport: Molecular mechanisms, genetic disorders, and treatment. A summary of the 1998 AASLD single topic conference. Hepatology 1998, 28: 1713-1719. PMID: 9828241, DOI: 10.1002/hep.510280637.Peer-Reviewed Original ResearchCyclic AMP stimulates sorting of the canalicular organic anion transporter (Mrp2/cMoat) to the apical domain in hepatocyte couplets
Roelofsen H, Soroka C, Keppler D, Boyer J. Cyclic AMP stimulates sorting of the canalicular organic anion transporter (Mrp2/cMoat) to the apical domain in hepatocyte couplets. Journal Of Cell Science 1998, 111: 1137-1145. PMID: 9512509, DOI: 10.1242/jcs.111.8.1137.Peer-Reviewed Original ResearchConceptsTransport proteinsApical membraneApical domainTransporter-containing vesiclesMultidrug resistance protein 2Protein 2Cell-cell contactMicrotubule-dependent fashionBasolateral plasma membraneApical proteinsEffect of cAMPVesicle trafficAnion transportersSecretory polarityOrganic anion transportersPlasma membraneCanalicular membraneAnion transport capacityTransport activityApical excretionApical poleCanalicular organic anion transportMultispecific organic anion transporterAmphiphilic organic anionsTransport function
1997
Enterohepatic circulation of scymnol sulfate in an elasmobranch, the little skate (Raja erinacea)
Fricker G, Wössner R, Drewe J, Fricker R, Boyer J. Enterohepatic circulation of scymnol sulfate in an elasmobranch, the little skate (Raja erinacea). American Journal Of Physiology 1997, 273: g1023-g1030. PMID: 9374698, DOI: 10.1152/ajpgi.1997.273.5.g1023.Peer-Reviewed Original ResearchConceptsEnterohepatic circulationScymnol sulfateBile alcohol sulfatesBile flowBiliary secretionCholeretic effectMajor bile saltsHepatic uptakeHepatic eliminationIntestinal epitheliumBileLiverSkate liverHepatic transportSpecific transport mechanismsBile alcoholsMajor metaboliteFirst direct demonstrationBile saltsCholyltaurinePerfusateLittle skatePolar metabolitesCompetitive mannerDirect demonstration
1996
Bile secretion-models, mechanisms, and malfunctions. A perspective on the development of modern cellular and molecular concepts of bile secretion and cholestasis
Boyer J. Bile secretion-models, mechanisms, and malfunctions. A perspective on the development of modern cellular and molecular concepts of bile secretion and cholestasis. Journal Of Gastroenterology 1996, 31: 475-481. PMID: 8726846, DOI: 10.1007/bf02355044.Peer-Reviewed Original ResearchConceptsBile secretionActive microtubule-dependent secretion of a fluorescent bile salt derivative in skate hepatocyte clusters
Miller D, Fricker G, Schramm U, Henson J, Hager D, Nundy S, Ballatori N, Boyer J. Active microtubule-dependent secretion of a fluorescent bile salt derivative in skate hepatocyte clusters. American Journal Of Physiology 1996, 270: g887-g896. PMID: 8764193, DOI: 10.1152/ajpgi.1996.270.6.g887.Peer-Reviewed Original ResearchRegulation of intracellular pH in the hepatocyte Mechanisms and physiological implications
Strazzabosco M, Boyer J. Regulation of intracellular pH in the hepatocyte Mechanisms and physiological implications. Journal Of Hepatology 1996, 24: 631-644. PMID: 8773922, DOI: 10.1016/s0168-8278(96)80153-x.Peer-Reviewed Original ResearchBile duct epithelium: frontiers in transport physiology
Boyer J. Bile duct epithelium: frontiers in transport physiology. American Journal Of Physiology 1996, 270: g1-g5. PMID: 8772494, DOI: 10.1152/ajpgi.1996.270.1.g1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBicarbonatesBileBile DuctsBiological TransportCystic Fibrosis Transmembrane Conductance RegulatorEpitheliumHumansModels, Biological
1994
Characteristics of bile salt uptake into skate hepatocytes
Fricker G, Dubost V, Finsterwald K, Boyer J. Characteristics of bile salt uptake into skate hepatocytes. Biochemical Journal 1994, 299: 665-670. PMID: 8192655, PMCID: PMC1138072, DOI: 10.1042/bj2990665.Peer-Reviewed Original ResearchConceptsOrganic anion transport systemBile salt uptakeBile alcoholsBile saltsBeta-cholestane-3 alphaBile salt transportUnconjugated bile saltsTaurine-conjugated bile saltsHepatic uptakeAlpha-triolSkate bileInhibitory effectLiver cellsSkate hepatocytesIndependent transport systemsCholyltaurine transportCholyltaurineHepatocytesInhibitionAlphaUptakeSpecificity
1993
Characterization of bumetanide transport in isolated skate hepatocytes
Blumrich M, Petzinger E, Boyer J. Characterization of bumetanide transport in isolated skate hepatocytes. American Journal Of Physiology 1993, 265: g926-g933. PMID: 8238522, DOI: 10.1152/ajpgi.1993.265.5.g926.Peer-Reviewed Original ResearchEffect of secretion on intracellular pH regulation in isolated rat bile duct epithelial cells.
Alvaro D, Cho W, Mennone A, Boyer J. Effect of secretion on intracellular pH regulation in isolated rat bile duct epithelial cells. Journal Of Clinical Investigation 1993, 92: 1314-1325. PMID: 8397224, PMCID: PMC288273, DOI: 10.1172/jci116705.Peer-Reviewed Original ResearchConceptsBile duct epithelial cellsDuct epithelial cellsCl-/HCO3Cell depolarizationEpithelial cellsEffect of secretinCl- channel blockerEffects of secretionsCl removalCl- channel activationRat bile duct epithelial cellsKRB mediumMaximal rateRegulation of intracellularBicarbonate secretionNormal rat liverIntracellular pH regulationMM DIDSHCO3- excretionAcid loadBDE cellsSecretinMicrofluorimetric methodRat liverHCO3- influx