mTOR Controls Ovarian Follicle Growth by Regulating Granulosa Cell Proliferation
Yu J, Yaba A, Kasiman C, Thomson T, Johnson J. mTOR Controls Ovarian Follicle Growth by Regulating Granulosa Cell Proliferation. PLOS ONE 2011, 6: e21415. PMID: 21750711, PMCID: PMC3130037, DOI: 10.1371/journal.pone.0021415.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsApoptosisBlotting, WesternCarrier ProteinsCell DivisionCell Line, TransformedCell ProliferationDose-Response Relationship, DrugFemaleFlow CytometryG1 PhaseGranulosa CellsImmunosuppressive AgentsMaleMiceMice, Inbred C57BLOvarian FolliclePhosphorylationRatsRegulatory-Associated Protein of mTORRibosomal Protein S6 Kinases, 70-kDaSirolimusTOR Serine-Threonine KinasesConceptsAberrant mitotic figuresGranulosa cellsFollicle growthMitotic figuresMice treated with rapamycinFraction of granulosa cellsEffects of mTOR inhibitionRegulation of follicle growthDose-dependent increaseDose-dependent reductionRegulating granulosa cell proliferationGranulosa cell proliferationReduced compared to controlsMTOR pathway activationAberrant mitotic eventsExpression of RaptorOvarian follicle growthOvarian folliclesInhibition of mTORPresence of rapamycinMTOR inhibitionGranulosaInhibitor rapamycinConsequences of increased numbersMitotic index