Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes
Wang Y, Yang J, Hu F, Yang Y, Huang K, Zhang K. Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes. Journal Of Cell Biology 2023, 222: e202301066. PMID: 37882754, PMCID: PMC10602792, DOI: 10.1083/jcb.202301066.Peer-Reviewed Original ResearchConceptsSingle-particle cryo-electron microscopy structureCryo-electron microscopy structureNon-polar filamentsImmunoglobulin-like domainsDistal regionPolyproline II helixMicroscopy structureChlamydomonas reinhardtiiFlagellar motilityVaried environmental conditionsCryo-EMCell swimmingMotility controlEnvironmental responsesDisulfide bondsEnvironmental conditionsSushi domainMastigonemesThread-like structuresRedox shiftSignal sensingPotential roleProtistsReinhardtiiMST1Abl2 repairs microtubules and phase separates with tubulin to promote microtubule nucleation
Duan D, Lyu W, Chai P, Ma S, Wu K, Wu C, Xiong Y, Sestan N, Zhang K, Koleske A. Abl2 repairs microtubules and phase separates with tubulin to promote microtubule nucleation. Current Biology 2023, 33: 4582-4598.e10. PMID: 37858340, PMCID: PMC10877310, DOI: 10.1016/j.cub.2023.09.018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MovementChlorocebus aethiopsCOS CellsMicrotubule-Associated ProteinsMicrotubulesTubulinConceptsCOS-7 cellsMT nucleationMT latticeFamily kinasesTubulin recruitmentLiquid-liquid phase separationTubulin C-terminal tailsCryo-EM analysisC-terminal tailAbl family kinasesWild-type cellsC-terminal halfRescue frequencyGenetic experimentsNeuronal morphogenesisMicrotubule nucleationSplice isoformsMicrotubule dynamicsNocodazole treatmentMolecular mechanismsAxon guidanceCell migrationDamage sitesABL2MT assembly