2016
Replication fork passage drives asymmetric dynamics of a critical nucleoid‐associated protein in Caulobacter
Arias‐Cartin R, Dobihal GS, Campos M, Surovtsev IV, Parry B, Jacobs‐Wagner C. Replication fork passage drives asymmetric dynamics of a critical nucleoid‐associated protein in Caulobacter. The EMBO Journal 2016, 36: 301-318. PMID: 28011580, PMCID: PMC5286365, DOI: 10.15252/embj.201695513.Peer-Reviewed Original ResearchConceptsNucleoid-associated proteinsDNA replicationNovel nucleoid-associated proteinGene expressionReplication fork passageGlobal gene expressionCell cycle regulationDNA-binding activityCell cycle progressionChromosome dynamicsFork passageChromosome segregationPleiotropic defectsChromosomal biasCaulobacter crescentusAsymmetric localizationReplication forksCellular functionsCycle regulationGenomic techniquesQuantitative cell imagingCell divisionGapRCycle progressionCell cycle
2014
A Constant Size Extension Drives Bacterial Cell Size Homeostasis
Campos M, Surovtsev IV, Kato S, Paintdakhi A, Beltran B, Ebmeier SE, Jacobs-Wagner C. A Constant Size Extension Drives Bacterial Cell Size Homeostasis. Cell 2014, 159: 1433-1446. PMID: 25480302, PMCID: PMC4258233, DOI: 10.1016/j.cell.2014.11.022.Peer-Reviewed Original ResearchConceptsCell size homeostasisCell size controlSize homeostasisSingle-cell microscopyCell cycle controlCaulobacter crescentusAsymmetric divisionHomeostasis mechanismsBacteria Escherichia coliCell cycleEscherichia coliDifferent growth ratesCell growthCell lengthSize controlCell sizeHomeostasisBacteriaSize thresholdGrowth rateDivisionDirect experimental evidenceCrescentusSize paradigmColiEvidence for a DNA-relay mechanism in ParABS-mediated chromosome segregation
Lim HC, Surovtsev IV, Beltran BG, Huang F, Bewersdorf J, Jacobs-Wagner C. Evidence for a DNA-relay mechanism in ParABS-mediated chromosome segregation. ELife 2014, 3: e02758. PMID: 24859756, PMCID: PMC4067530, DOI: 10.7554/elife.02758.Peer-Reviewed Original ResearchConceptsChromosome segregationPartition complexParA-like proteinBacterial chromosome segregationDNA-binding activityParABS systemCytoplasmic cargoCaulobacter crescentusDNA regionsBiochemical approachesIntracellular transportTranslocation forceCellular parametersChromosomesTransient tethersCrescentusParABSMajor roleSegregationComplexesMechanismProteinTranslocationConservationBacteria
2013
The Bacterial Cytoplasm Has Glass-like Properties and Is Fluidized by Metabolic Activity
Parry BR, Surovtsev IV, Cabeen MT, O’Hern C, Dufresne ER, Jacobs-Wagner C. The Bacterial Cytoplasm Has Glass-like Properties and Is Fluidized by Metabolic Activity. Cell 2013, 156: 183-194. PMID: 24361104, PMCID: PMC3956598, DOI: 10.1016/j.cell.2013.11.028.Peer-Reviewed Original ResearchMeSH KeywordsBiophysical PhenomenaCaulobacter crescentusChromosomes, BacterialCytoplasmEscherichia coliPlasmids
2010
Spatial organization of the flow of genetic information in bacteria
Montero Llopis P, Jackson AF, Sliusarenko O, Surovtsev I, Heinritz J, Emonet T, Jacobs-Wagner C. Spatial organization of the flow of genetic information in bacteria. Nature 2010, 466: 77-81. PMID: 20562858, PMCID: PMC2896451, DOI: 10.1038/nature09152.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCaulobacter crescentusChaperoninsChromosomes, BacterialDiffusionDNA, BacterialEndoribonucleasesEscherichia coliGene Expression Regulation, BacterialIn Situ Hybridization, FluorescenceLac OperonProtein BiosynthesisRibosomesRNA StabilityRNA TransportRNA, BacterialRNA, MessengerTranscription, GeneticConceptsSites of transcriptionC. crescentusCaulobacter crescentusEukaryotic cellsCellular physiologyMRNA decayMature mRNAMRNA processesRNase EMRNA substratesMRNA localizationGenetic informationGene expressionBacterial cellsEscherichia coliQuantitative fluorescenceCrescentusSitu hybridizationSpatial organizationMRNABacteriaLimited dispersionCellsTranscriptionTranslation