2021
Interconnecting solvent quality, transcription, and chromosome folding in Escherichia coli
Xiang Y, Surovtsev IV, Chang Y, Govers SK, Parry BR, Liu J, Jacobs-Wagner C. Interconnecting solvent quality, transcription, and chromosome folding in Escherichia coli. Cell 2021, 184: 3626-3642.e14. PMID: 34186018, DOI: 10.1016/j.cell.2021.05.037.Peer-Reviewed Original Research
2018
Subcellular Organization: A Critical Feature of Bacterial Cell Replication
Surovtsev IV, Jacobs-Wagner C. Subcellular Organization: A Critical Feature of Bacterial Cell Replication. Cell 2018, 172: 1271-1293. PMID: 29522747, PMCID: PMC5870143, DOI: 10.1016/j.cell.2018.01.014.Peer-Reviewed Original ResearchConceptsSelf-replicating entitiesCellular lifeStructured genomesDynamic spatial patternsChallenge cellsIntracellular organellesCellular replicationCytoskeletal filamentsBacterial cellsUnique biological propertiesComplex internal organizationSpatial organizationSmall formsCell replicationActive transportLiving systemsBacteriaReplicationGenomeBiological propertiesCellsSpatial patternsOrganellesPowerful meansHallmark
2016
DNA-relay mechanism is sufficient to explain ParA-dependent intracellular transport and patterning of single and multiple cargos
Surovtsev IV, Campos M, Jacobs-Wagner C. DNA-relay mechanism is sufficient to explain ParA-dependent intracellular transport and patterning of single and multiple cargos. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: e7268-e7276. PMID: 27799522, PMCID: PMC5135302, DOI: 10.1073/pnas.1616118113.Peer-Reviewed Original ResearchConceptsParA ATPase activityParA gradientCellular physiologyATP bindingBinds DNAIntracellular patterningChromosomal lociATP hydrolysisIntracellular transportInside cellsMultiple copiesMultiple cargoesDNA fluctuationsATPase activityIntracellular patternMacromolecular componentsCargoDNAPatterningMinimal systemBindingSignature patternsParBCargo dynamicsCells
2010
Spatial organization of the flow of genetic information in bacteria
Montero Llopis P, Jackson AF, Sliusarenko O, Surovtsev I, Heinritz J, Emonet T, Jacobs-Wagner C. Spatial organization of the flow of genetic information in bacteria. Nature 2010, 466: 77-81. PMID: 20562858, PMCID: PMC2896451, DOI: 10.1038/nature09152.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCaulobacter crescentusChaperoninsChromosomes, BacterialDiffusionDNA, BacterialEndoribonucleasesEscherichia coliGene Expression Regulation, BacterialIn Situ Hybridization, FluorescenceLac OperonProtein BiosynthesisRibosomesRNA StabilityRNA TransportRNA, BacterialRNA, MessengerTranscription, GeneticConceptsSites of transcriptionC. crescentusCaulobacter crescentusEukaryotic cellsCellular physiologyMRNA decayMature mRNAMRNA processesRNase EMRNA substratesMRNA localizationGenetic informationGene expressionBacterial cellsEscherichia coliQuantitative fluorescenceCrescentusSitu hybridizationSpatial organizationMRNABacteriaLimited dispersionCellsTranscriptionTranslation
2009
Mathematical modeling of a minimal protocell with coordinated growth and division
Surovtsev I, Zhang Z, Lindahl P, Morgan J. Mathematical modeling of a minimal protocell with coordinated growth and division. Journal Of Theoretical Biology 2009, 260: 422-429. PMID: 19501600, DOI: 10.1016/j.jtbi.2009.06.001.Peer-Reviewed Original ResearchConceptsCellular componentsDaughter cellsWhole-cell modeling frameworkParental cellsCell cycle engineMinimal protocellWhole-cell modelsContractile ringCoordinated growthCell shapeCell cycleCellular geometryMembrane areaProtocellsMolecular-level mechanismsCellsSimultaneous doublingCytokinesisCell volume
2004
Laminin-binding protein as a cellular receptor for the equine Venezuelan encephalomyelitis virus: Report 2. Inhibition of replication of equine Venezuelan encephalomyelitis virus by blocking laminin-binding protein on the surface of Vero cells.
Bondarenko E, Protopopova E, Konovalova S, Surovtsev I, Mal'tsev V, Loktev V. Laminin-binding protein as a cellular receptor for the equine Venezuelan encephalomyelitis virus: Report 2. Inhibition of replication of equine Venezuelan encephalomyelitis virus by blocking laminin-binding protein on the surface of Vero cells. Molecular Genetics, Microbiology And Virology 2004, 36-40. PMID: 15025003.Peer-Reviewed Original ResearchConceptsLaminin-binding proteinVero cellsVirus replicationEncephalomyelitis virusVEE virusVEE virus infectionVirion penetrationVirus infectionFITC-labeled antibodiesRabbit polyclonal antibodyVero cell surfaceInhibition of replicationRecombinant LBPCell surfaceCellular receptorsVirusPossible inhibitionSuch cellsPolyclonal antibodiesReport 2Scanning cytometryAntibodiesCellsInhibition