2007
Nickel-Dependent Oligomerization of the Alpha Subunit of Acetyl-Coenzyme A Synthase/Carbon Monoxide Dehydrogenase †
Tan X, Kagiampakis I, Surovtsev I, Demeler B, Lindahl P. Nickel-Dependent Oligomerization of the Alpha Subunit of Acetyl-Coenzyme A Synthase/Carbon Monoxide Dehydrogenase †. Biochemistry 2007, 46: 11606-11613. PMID: 17887777, PMCID: PMC2528952, DOI: 10.1021/bi7014663.Peer-Reviewed Original ResearchConceptsAlpha subunitCarbon monoxide dehydrogenaseSynthase/carbon monoxide dehydrogenaseApo-alphaHigh molecular weight speciesMolecular weight speciesRecombinant alpha subunitAcetyl-CoA synthase/carbon monoxide dehydrogenaseWeight speciesOpen conformationSulfur proteinA-clusterConformational changesSubunitsStructural scaffoldOligomerizationFe4S4 clusterSpeciesActive siteConformationDehydrogenaseInterrelated eventsActivationOligomerizesDimers
2006
Kinetics of CO Insertion and Acetyl Group Transfer Steps, and a Model of the Acetyl-CoA Synthase Catalytic Mechanism
Tan X, Surovtsev I, Lindahl P. Kinetics of CO Insertion and Acetyl Group Transfer Steps, and a Model of the Acetyl-CoA Synthase Catalytic Mechanism. Journal Of The American Chemical Society 2006, 128: 12331-12338. PMID: 16967985, PMCID: PMC2527582, DOI: 10.1021/ja0627702.Peer-Reviewed Original ResearchConceptsCO insertionCatalytic mechanismGroup transferNiFeC EPR signalAcetyl-CoA synthase/carbon monoxide dehydrogenaseEquilibrium constantsMethyl groupSynthase/carbon monoxide dehydrogenaseCO insertion stepInsertion of COCarbon monoxide dehydrogenaseSubsequent catalytic stepsActive enzyme moleculesStopped-flow methodTransfer stepIndividual equilibrium constantsInsertion stepMethyl group transferEPR signalEnzyme moleculesCatalytic stepSimple kinetic modelSulfur proteinVariation of activityRate coefficients