2021
Next-Generation Sequencing for Pathogen Identification in Infected Foot Ulcers
Choi Y, Oda E, Waldman O, Sajda T, Beck C, Oh I. Next-Generation Sequencing for Pathogen Identification in Infected Foot Ulcers. Foot & Ankle Orthopaedics 2021, 6: 24730114211026933. PMID: 35097461, PMCID: PMC8702686, DOI: 10.1177/24730114211026933.Peer-Reviewed Original ResearchDiabetic foot ulcersFoot infectionsFoot ulcersNext-generation sequencingOrthopedic infectionsInfected diabetic foot ulcersFellowship-trained orthopedic footPostoperative antibiotic treatmentInfected foot ulcersStandard cultureConventional cultureWarrants further investigationManagement of footDFU patientsOrthopaedic FootOperative treatmentKnee amputationAntibiotic treatmentRay amputationOperative procedureAnkle surgeonsCommon pathogensAverage ageCommon microorganismsConcordance rateComparative study of culture, next‐generation sequencing, and immunoassay for identification of pathogen in diabetic foot ulcer
Lipof JS, Jones CMC, Daiss J, Oh I. Comparative study of culture, next‐generation sequencing, and immunoassay for identification of pathogen in diabetic foot ulcer. Journal Of Orthopaedic Research® 2021, 39: 2638-2645. PMID: 33543785, PMCID: PMC8339135, DOI: 10.1002/jor.25001.Peer-Reviewed Original ResearchConceptsDiabetic foot infectionsMusculoskeletal infectionsNext-generation sequencingS. agalactiaeS. aureusCulture-directed antibioticsDeep musculoskeletal infectionNegative initial culturesProspective observational studyTime of diagnosisDiabetic foot ulcersLimb salvage effortsPeripheral blood samplesYears of ageFoot infectionsSurgical debridementFoot ulcersRecurrent infectionsTreatment successFair correlationIntraoperative samplesObservational studyBlood samplesUseful adjunctOrthopaedic surgeons
2020
Species-Specific Immunoassay Aids Identification of Pathogen and Tracks Infectivity in Foot Infection
Hao SP, Masters EA, Ninomiya MJ, Beck CA, Schwarz EM, Daiss JL, Oh I. Species-Specific Immunoassay Aids Identification of Pathogen and Tracks Infectivity in Foot Infection. Foot & Ankle International 2020, 42: 363-372. PMID: 33161780, PMCID: PMC7946706, DOI: 10.1177/1071100720965136.Peer-Reviewed Original ResearchConceptsFoot infectionsHost immune responseWound healing statusImmune responseHealing statusFoot ulcer patientsProspective comparative studyCulture-positive patientsCulture-negative patientsSpecific serum antibodiesDominant pathogenAnalysis of serumConventional bacterial cultureUlcer patientsMusculoskeletal infectionsSurgical interventionAntibody titersSerum antibodiesTherapeutic responseInfection diagnosisSpecies-specific immunoassaySurgeon's decisionCulture resultsInfectionLevel IIChopart Amputation: Questioning the Clinical Efficacy of a Long-standing Surgical Option for Diabetic Foot Infection.
Brodell JD, Ayers BC, Baumhauer JF, DiGiovanni BF, Flemister AS, Ketz JP, Oh I. Chopart Amputation: Questioning the Clinical Efficacy of a Long-standing Surgical Option for Diabetic Foot Infection. Journal Of The American Academy Of Orthopaedic Surgeons 2020, 28: 684-691. PMID: 32769724, DOI: 10.5435/jaaos-d-19-00757.Peer-Reviewed Original ResearchConceptsBaseline physical functionPROMIS Physical FunctionDiabetic foot infectionsPostoperative wound complicationsChopart amputationRevision amputationFoot infectionsWound complicationsPhysical functionWound healingOutcomes Measurement Information System (PROMIS) instrumentsPROMIS physical function scoresPhysical function scoresPatient-reported outcomesJudicious patient selectionExtent of infectionPostoperative complicationsTotal patientsCase seriesClinical outcomesKnee amputationPain interferencePatient selectionSurgical optionsTendon transfer
2019
Should Chopart Amputation be Performed in Diabetic Foot Ulcer Patients?
Brodell J, Baumhauer J, DiGiovanni B, Flemister A, Ketz J, Oh I. Should Chopart Amputation be Performed in Diabetic Foot Ulcer Patients? Foot & Ankle Orthopaedics 2019, 4: 2473011419s00118. PMCID: PMC8696696, DOI: 10.1177/2473011419s00118.Peer-Reviewed Original ResearchChopart amputationSyme amputationAmputation patientsRevision amputationSF-36Patient cohortExact testWound healingOutcomes Measurement Information System (PROMIS) instrumentsDiabetic foot ulcer patientsMinor post-operative complicationsFoot ulcer patientsCurrent Procedural Terminology codesDiabetic foot infectionsPost-operative complicationsBelow knee amputationBody mass indexJudicious patient selectionUnpaired Student's t-testProcedural Terminology codesFisher's exact testExtent of infectionStudent's t-testFoot infectionsMedical comorbidities
2018
Tracking Anti-Staphylococcus aureus Antibodies Produced In Vivo and Ex Vivo during Foot Salvage Therapy for Diabetic Foot Infections Reveals Prognostic Insights and Evidence of Diversified Humoral Immunity
Oh I, Muthukrishnan G, Ninomiya MJ, Brodell JD, Smith BL, Lee CC, Gill SR, Beck CA, Schwarz EM, Daiss JL. Tracking Anti-Staphylococcus aureus Antibodies Produced In Vivo and Ex Vivo during Foot Salvage Therapy for Diabetic Foot Infections Reveals Prognostic Insights and Evidence of Diversified Humoral Immunity. Infection And Immunity 2018, 86: 10.1128/iai.00629-18. PMID: 30275008, PMCID: PMC6246899, DOI: 10.1128/iai.00629-18.Peer-Reviewed Original ResearchConceptsDiabetic foot infectionsIgG levelsDFI patientsSalvage therapyFoot infectionsCultured peripheral blood mononuclear cellsPeripheral blood mononuclear cellsBlood mononuclear cellsSimilar diagnostic potentialAggressive treatmentAntibody titersHumoral immunityMononuclear cellsPrognostic insightsPrognostic potentialCommon pathogensEtiologic agentMultivariate analysisClinical decisionEx vivoMultiplex immunoassayWound samplesInfectionSerumAureus antibody
2017
Clinical Application of Immunoassay for Identification of Staphylococcus aureus and Monitoring Treatment Response in Diabetic Foot Infection
Oh I, Smith B, Grier A, Gill S, Daiss J. Clinical Application of Immunoassay for Identification of Staphylococcus aureus and Monitoring Treatment Response in Diabetic Foot Infection. Foot & Ankle Orthopaedics 2017, 2: 2473011417s000065. DOI: 10.1177/2473011417s000065.Peer-Reviewed Original ResearchDiabetic foot infectionsFoot infectionsSA infectionsAntibiotic treatmentTreatment responseStaphylococcus aureusPathogenic activityAdequate prognostic informationNonspecific inflammatory markersIntravenous antibiotic treatmentWhole bloodMonitoring Treatment ResponseSigns of infectionStandard cultureAntibody-secreting cellsMicrobiome analysisFoot salvageSalvage therapySalvage treatmentSpecific diagnostic toolsInflammatory markersDiabetic patientsCounseling patientsClinical symptomsWeeks 0