Using Cloning to Amplify Neuronal Genomes for Whole-Genome Sequencing and Comprehensive Mutation Detection and Validation
Hazen J, Duran M, Smith R, Rodriguez A, Martin G, Kupriyanov S, Hall I, Baldwin K. Using Cloning to Amplify Neuronal Genomes for Whole-Genome Sequencing and Comprehensive Mutation Detection and Validation. Neuromethods 2017, 131: 163-185. DOI: 10.1007/978-1-4939-7280-7_9.Peer-Reviewed Original ResearchNeuronal genomeSomatic cell nuclear transferEmbryonic cell lineClasses of mutationsEmbryonic stem cellsSequencing-based approachesCell nuclear transferSomatic mutationsWhole-genome sequencingNext-generation sequencingMutation detectionSomatic cellsBioinformatics methodsComprehensive mutation detectionNuclear transferIndividual mutationsGenomeCell typesStem cellsNew mutationsMutationsSingle cellsSequencingCell linesFalse positive calls