2017
Development of an inducible platform for intercellular protein delivery
Siller R, Dufour E, Lycke M, Wilmut I, Jung YW, Park IH, Sullivan GJ. Development of an inducible platform for intercellular protein delivery. International Journal Of Pharmaceutics 2017, 522: 1-10. PMID: 28254654, DOI: 10.1016/j.ijpharm.2017.02.067.Peer-Reviewed Original ResearchConceptsNovel platformBiomolecules of interestCell penetrating peptideGene of interestHuman chorionic gonadotropin beta subunitProtein deliveryStem cell biologyChorionic gonadotropin beta subunitPenetrating peptidePotential applicationsProtein transduction domainProtein of interestGreen fluorescent proteinFusion proteinGonadotropin beta subunitEfficient uptakeN-terminal signal peptideCell culture mediumPlatformFluorescent proteinTransduction domainLack of specificityBeta subunitActive proteinFixation process
2011
Gene-Correction Rescues Reprogramming of Fanconi Anemia Fibroblasts and Enables Hematopoietic Differentiation of FA Induced Pluripotent Stem Cells in Vitro and In Vivo
Mueller L, Milsom M, Harris C, Vyas R, Brumme K, Parmar K, Schambach A, Grassman E, Park I, Wendy L, Strait K, Schlaeger T, Devine A, D'Andrea A, Daley G, Williams D. Gene-Correction Rescues Reprogramming of Fanconi Anemia Fibroblasts and Enables Hematopoietic Differentiation of FA Induced Pluripotent Stem Cells in Vitro and In Vivo. Blood 2011, 118: 672. DOI: 10.1182/blood.v118.21.672.672.Peer-Reviewed Original ResearchFA cellsFA pathwayFanconi anemiaPluripotent stem cellsHematopoietic differentiationGenomic instabilityStem cellsIPSC linesDouble-strand DNA breaksHematopoietic cellsFANCD2 foci formationPatient iPSC linesHematopoietic differentiation potentialFanconi anemia fibroblastsTail-tip fibroblastsInduced pluripotent stem cellsDefective DNA repairGreen fluorescent proteinFatal bone marrow failureGenomic integritySignificant chromosomal imbalancesHematopoietic stem cellsComparative genomic hybridizationComplementation groupsDNA repair
2008
Patient-Specific Induced Pluripotent Stem Cells in Hurler Syndrome
Tolar J, Park I, Xia L, Osborn M, McElmurry R, Orchard P, Daley G, Blazar B. Patient-Specific Induced Pluripotent Stem Cells in Hurler Syndrome. Blood 2008, 112: 386. DOI: 10.1182/blood.v112.11.386.386.Peer-Reviewed Original ResearchGreen fluorescent proteinPluripotent stem cellsIPS cellsStem cellsMesenchymal stromal cellsTumor rejection antigen-1Such induced pluripotent stem cellsWild-type levelsAutologous pluripotent cellsTransient mRNA expressionInduced pluripotent stem cellsMouse embryo fibroblastsStage-specific embryonic antigen-3Adult stem cellsEmbryonic stem cell isolationHematopoietic progenyHematopoietic stem cellsStable transgenesisTransposon insertionMesodermal lineagesNumerous tissue typesSleeping Beauty transposon systemTranscription factorsNon-viral Sleeping Beauty (SB) transposon systemPluripotent cells