2022
Mapping subcellular localizations of unannotated microproteins and alternative proteins with MicroID
Na Z, Dai X, Zheng SJ, Bryant CJ, Loh KH, Su H, Luo Y, Buhagiar AF, Cao X, Baserga SJ, Chen S, Slavoff SA. Mapping subcellular localizations of unannotated microproteins and alternative proteins with MicroID. Molecular Cell 2022, 82: 2900-2911.e7. PMID: 35905735, PMCID: PMC9662605, DOI: 10.1016/j.molcel.2022.06.035.Peer-Reviewed Original ResearchConceptsSubcellular localizationProximity biotinylationSmall open reading framesAlternative proteinsOpen reading frameHigh-throughput technologiesSubnuclear organellesCanonical proteinsRRNA transcriptionSubcellular compartmentsReading frameProteogenomic identificationProtein compositionAmino acidsMicroproteinsProteinBiotinylationLocalizationTurboIDTranscriptionOrganellesMouse modelPolypeptideNucleoliExpression
2020
A leptin-BDNF pathway regulating sympathetic innervation of adipose tissue.
Wang P, Loh KH, Wu M, Morgan DA, Schneeberger M, Yu X, Chi J, Kosse C, Kim D, Rahmouni K, Cohen P, Friedman J. A leptin-BDNF pathway regulating sympathetic innervation of adipose tissue. Nature 2020, 583: 839-844. PMID: 32699414, DOI: 10.1038/s41586-020-2527-y.Peer-Reviewed Original Research
2017
In Situ Peroxidase Labeling and Mass-Spectrometry Connects Alpha-Synuclein Directly to Endocytic Trafficking and mRNA Metabolism in Neurons
Chung CY, Khurana V, Yi S, Sahni N, Loh KH, Auluck PK, Baru V, Udeshi ND, Freyzon Y, Carr SA, Hill DE, Vidal M, Ting AY, Lindquist S. In Situ Peroxidase Labeling and Mass-Spectrometry Connects Alpha-Synuclein Directly to Endocytic Trafficking and mRNA Metabolism in Neurons. Cell Systems 2017, 4: 242-250.e4. PMID: 28131823, PMCID: PMC5578869, DOI: 10.1016/j.cels.2017.01.002.Peer-Reviewed Original Research
2016
A human microprotein that interacts with the mRNA decapping complex
D'Lima NG, Ma J, Winkler L, Chu Q, Loh KH, Corpuz EO, Budnik BA, Lykke-Andersen J, Saghatelian A, Slavoff SA. A human microprotein that interacts with the mRNA decapping complex. Nature Chemical Biology 2016, 13: 174-180. PMID: 27918561, PMCID: PMC5247292, DOI: 10.1038/nchembio.2249.Peer-Reviewed Original ResearchProteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts.
Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY. Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts. Cell 2016, 166: 1295-1307.e21. PMID: 27565350, PMCID: PMC5167540, DOI: 10.1016/j.cell.2016.07.041.Peer-Reviewed Original ResearchSpatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2.
Hung V, Udeshi ND, Lam SS, Loh KH, Cox KJ, Pedram K, Carr SA, Ting AY. Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2. Nature Protocols 2016, 11: 456-75. PMID: 26866790, PMCID: PMC4863649, DOI: 10.1038/nprot.2016.018.Peer-Reviewed Original Research
2013
Imaging trans-cellular neurexin-neuroligin interactions by enzymatic probe ligation.
Liu DS, Loh KH, Lam SS, White KA, Ting AY. Imaging trans-cellular neurexin-neuroligin interactions by enzymatic probe ligation. PloS One 2013, 8: e52823. PMID: 23457442, PMCID: PMC3573046, DOI: 10.1371/journal.pone.0052823.Peer-Reviewed Original Research
2012
Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
Liu DS, Phipps WS, Loh KH, Howarth M, Ting AY. Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells. ACS Nano 2012, 6: 11080-7. PMID: 23181687, PMCID: PMC3528850, DOI: 10.1021/nn304793z.Peer-Reviewed Original Research