3D structure and in situ arrangements of CatSper channel in the sperm flagellum
Zhao Y, Wang H, Wiesehoefer C, Shah NB, Reetz E, Hwang JY, Huang X, Wang TE, Lishko PV, Davies KM, Wennemuth G, Nicastro D, Chung JJ. 3D structure and in situ arrangements of CatSper channel in the sperm flagellum. Nature Communications 2022, 13: 3439. PMID: 35715406, PMCID: PMC9205950, DOI: 10.1038/s41467-022-31050-8.Peer-Reviewed Original ResearchConceptsCatSper complexSperm flagellaCryo-electron tomographyHigher-order organizationMammalian spermIntracellular domainStructural basisExtracellular domainTetrameric channelChannel CatSperSuccessful fertilizationFlagellar movementCatSper channelsChannel poreSitu mapSitu arrangementHuman sperm flagellumPrimary Ca2CatSperDimer formationFlagellaCentral roleSpermAtomic modelSperm motilityRedox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice
Wang H, Dou Q, Jeong KJ, Choi J, Gladyshev VN, Chung JJ. Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice. Journal Of Biological Chemistry 2022, 298: 102077. PMID: 35643315, PMCID: PMC9218152, DOI: 10.1016/j.jbc.2022.102077.Peer-Reviewed Original ResearchConceptsRedox regulationRedox homeostasisMolecular mechanismsSperm maturationMitochondrial sheath formationCell imaging analysisThioredoxin glutathione reductaseSperm developmentMammalian spermatozoaEpididymal transitMitochondrial ultrastructureMitochondrial activityRedox statusMale fertilityCapacitationHomeostasisMaturationRegulationMotilityFamily membersFertilizationSperm morphologySperm motilityMitochondriaBioenergetics